AccuPower® RocketScript™ RT PreMix, RNase H Minus uses RocketScript™ RTase and RNase H Minus developed by Bioneer independently. By using unique genetic engineering technology, it is useful for cDNA synthesis of long target RNA by removing RNase H activity. It is excellent in extensibility and sensitivity, and can successfully synthesize cDNA even in 1 pg human total RNA. It also contains all the components necessary for cDNA synthesis, so RT-reactions can be performed directly by adding RNA to be amplified and D.W.

Features and Benefits

• Rapid Synthesis Short-Time Synthesis : 9 kb synthesis possible with 10 minute RT reaction
  Time-efficient and economical synthesis of cDNA from secondary structure RNA with RocketScript™ RNase having excellent thermal stability capable of being active at even 70 °C
• No RNase H activity
  Useful for cDNA synthesis up to 12.5 kb from long target RNA using enzymes developed by proprietary genetic engineering technology
• Sensitivity
  High sensitivity and effective synthesis of cDNA even at scarce amount of 1 pg of human total RNA.
• Convienance
  Synthesis of cDNA samples difficult to be done using traditional methods, such as those made with long size RNA (9 kb), possible only within 10 minutes
• Reproducibility
  Minimized deviation between lots, then supplied as uniform quality product to provide reproducible experimental results by mass production under ISO 9001 quality system

Application

• First-strand synthesis of cDNA from RNA molecules (RT)
• Random priming reaction
• cDNA library construction
• Probe labeling
• mRNA 5'-end mapping by primer extension analysis
• PCR
• Real-time qPCR