Specifications
5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: Yes
Fragment Size: ~1 kb
Application
| Product Name |
5' to 3' exonuclease |
| Human and Animal |
STR analysis for determining genetic profiles in forensic cases |
| Molecular diagnostic analysis |
| Genotyping assay |
| Qualitative and semi-qualitative gene expression assay |
| Mutant screening |
| Transgenic organism analysis |
Figure 1. Single PCR and Multiplex PCR using AccuPower® Multiplex PCR PreMix
Each lane from left to right indicates the single and Multiplex PCR product using AccuPower® Multiplex PCR PreMix.
a) 10plex Multiplex PCR, b) 20plex Multiplex PCR
M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020)
Figure 2. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit.
6-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit. A series of Human genomic DNA diluents were tested. ( Lane 1. Human genomic DNA 100ng, Lane 2. Human genomic DNA 10ng, Lane 3. Human genomic DNA 1ng ). All data were obtained using Mygene 96 machine (Applied Bioneer co.)
Supplier Q : Q company Multiplex PCR kit, Supplier S : S company Multiplex PCR kit, Supplier I : I company Taq polymerase for Mutiplex PCR (0.5U, added 2mM MgCl2) Rxn. Condition : 95°C for 10min, followed by 35cycles of 30sec at 95°C, 30sec at 60°C, 60sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020)
Figure 3. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit.
10-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit. A series of Human genomic DNA diluents were tested. ( Lane 1. Human genomic DNA 100ng, Lane 2. Human genomic DNA 10ng, Lane 3. Human genomic DNA 1ng ). All data were obtained using Mygene 96 machine (Applied Bioneer co.)
Supplier Q : Q company Multiplex PCR kit, Supplier S : S company Multiplex PCR kit, Supplier I : I company Taq polymerase for Mutiplex PCR (0.5U, added 2mM MgCl2)Rxn. Condition : 95°C for 10min, followed by 35cycles of 30sec at 95°C, 30sec at 65°C, 60sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020)
Figure 4. Comparison of amplification quality using Lapchip® between AccuPower® Multiplex PCR PreMix and other supplier’s master mixture.
a) Virtual Gel Image. Gel image illustrates data reproducibility of the LabChip 90 system.
b) Overlay of expression level using Bioneer’s multiplex PCR PreMix and other company’s multiplex PCR kit. The electropherogram displays the data between 10 PCR products yield using 10-plex primer sets to illustrate the Amplification efficiency.
c) The graph shows the total concentration of PCR products between AccuPower® Multiplex PCR PreMix and other supplier’s master mixture.
Figure 5. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit.
20-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit. A series of Human genomic DNA diluents were tested. (100ng ~ 1ng). All data were obtained using Mygene 96 machine (Applied Bioneer co.)
Supplier Q : Q company Multiplex PCR kit, Supplier S : S company Multiplex PCR kit, Supplier I : I company Taq polymerase for Mutiplex PCR (0.5U, added 2mM MgCl2)
Rxn. Condition : 95°C for 10min, followed by 35cycles of 30sec at 95°C, 30sec at 57°C, 60sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020) Trademark: 'AccuPower®' is a registered trademark of Bioneer Corporation.