The world's first fully automated protein synthesis and nucleic acid extraction system

Function

Feature	Benefit
Fast Protein production	From DNA to Pure protein in about 6 hours
DNA purification	A wide variety of gDNA kits available
RNA purification	A wide variety of RNA kits available
Affinity protein purification	Sample preparation for bio-defense detection of various bacteria and viruses

Applications

Enzyme engineering, protein evolution, Synthetic biology, Bio-energy R&D, Protein drug R&D, expression screening, functional analysis, assay development and antigen production.

Specifications

Dimensions	320 mm(W) x 500 mm(H) x 535 mm(D) 12.6 in(W) x 19.7 in(H) x 21.1 in(D)
Weight	27 kg(59.5 lbs)
Temperature range	15 - 30 °C(59 - 86 °F)
Humidity range	20 - 80%, no condensation
Operating system	Built-in
User interface	320 x 240 touch screen graphic LCD
Input Voltage	100 - 240 VAC
Frequency	50 / 60 Hz
UV sterilization	15 minutes cycle
Communications	TCP/IP
Heat block	40 - 90 °C(104 - 194 °F)
Cooling block	4 °C(39.2 °F)

Functions

	ExiProgen™
Protein Expression	Yes
Protein Purification	Yes
DNA/RNA extraction	Yes
TFT Touch Screen	3.5"
Sample Number	1-16 samples
UV sterilization	Yes
Heated magnetic block	Yes
Contamination Shield	Yes
TCP/IP network Connection	Multiple systems can be daisy-chained together (up to 6)
Cooling system for sample storeage	Yes
Automatic dispensing	Yes
Power supply	24 VDC, 7.5 A(180 W)

Experimental Data

1. Protein expression and purification
- Amount of input DNA: 6 - 10 µg (purity >1.8)
- Protocol number : 902
- Total run time : < 6 h
- Yield: ~100 µg

Procedure

SDS-PAGE analysis

	Figure 1. GFP protein (His-tagged protein)’s expression/purification with ExiProgen™. M; AccuLadder™ Protein Size Maker (Low) E; expression sample P; purification sample
	Figure 2. Various His-tagged proteins expressed/purified with ExiProgen™. M; AccuLadder™ Protein Size Maker (Low) E; expression sample P; purification sample

2. Total RNA Extraction from Tissue or cultured mammalian cell
- Sample: 15 ㎎ (Rat liver tissue) or 1 X 10⁶ cells (HeLa)
- Protocol number: 202
- Average yield: 25 µg (Rat liver tissue), 10 µg (HeLa)
- Average RQS: > 9.5

* RQS(RNA quality score): The Caliper RQS is a calculated score that rates the quality of RNA samples. The RQS has been validated to correlate well with Agilent’s RIN (RNA Integrity Number) and follows the same 0-10 scale rating. Results comparing RIN to RQS for the same samples run on both LabChip GX and Agilent's Bioanalyzer 2100 typically show <10% deviation ( http://www.caliperls.jp/assets/pdf/lcgx/lcgx-AP-402.pdf)

Procedure

Rat total RNA Extracted from tissue


Total RNA from Rat was observed to contain intact rRNA with no evidence of degradation.

Real-Time PCR carried out on Rat Total RNA

Real time RT-PCR was carried out.AccuPower® RocketScrpt™ RT PreMix(K-2101, Bioneer), and AccuPower® DualStar™ qPCR PreMix(K-6110, Bioneer) , and the Exicycler™ 96 Real-Time PCR system (A-2060).

Analysis of total RNA from HeLa cells.

Total RNA was extracted from HeLa cell (1 X 10⁶ cells) and analyzed using the LabChip GX (Caliper Life Science). Each subunit of rRNA is clearly seen with no detectable degradation . In addition, the average yield of Total RNA was 10 µg, and the average RQS was 9.5 or higher.

Real-Time PCR carried out on total RNA from Hela cells.

Real time RT-PCR was carried out on total RNA extracted from HeLa cell (1 X 10⁶ cells) using AccuPower® RocketScrpt™ RT PreMix(K-2101, Bioneer), AccuPower® DualStar™ qPCR PreMix(K-6110, Bioneer) and the Exicycler™ 96 Real-Time PCR system(A-2060)

3. Genomic DNA from Cultured mammalian cell (HeLa))
- Sample volume: 1 X 10⁶ cells
- Protocol number: 107
- Total prep time: < 1 hr
- Average yield: 4-8 µg

Procedure

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X10⁶ cells of cultured HeLa cell and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH₂O as a negative control in DNA extraction. Note all samples have very similar yields.
Purity was also tested and was consistently between 1.8 and 2.0 (not shown).

4. Genomic DNA from Bacteria (E.coli)
- Sample volume: 1 X 10⁶ cells
- Protocol number: 109
- Total prep time: < 1 hr
- Average yield: 4-8 µg

Procedure

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X10⁶ cells of E.coli cells and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH₂O as a negative control in DNA extraction. Note all samples have very similar yields.
Purity was also tested and was consistently between 1.9 and 2.0 (not shown).

5. Genomic DNA from Tissue (Bovine skeletal muscle)
- Sample volume: 1 X 10⁶ cells
- Protocol number: 109
- Total prep time: < 1 hr
- Average yield: 8-12 µg

Procedure

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 20 mg of bovine tissues and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH₂O as a negative control in DNA extraction. Note all samples have very similar yields.
Purity was also tested and was consistently between 1.9 and 2.0 (not shown).

6. Genomic DNA from Plant Tissue (Spinach leaf)
- Sample volume: 100 ㎎
- Protocol number: 104
- Total prep time: <1 hr
- Average yield: 3-5 µg

Procedure

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 100 mg of spinach leaves and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH₂O as a negative control in DNA extraction. Note all samples have very similar yields.
Purity was also tested and was consistently between 1.9 and 2.0 (not shown).