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MagListo™ His-tagged Protein Purification Kit from Bioneer

Fast micro scale purification of 6x His-tagged protein

Overview
Technical/Specs
Literature/Support
Ordering Information


Application

figure1

Figure 1. Comparison of Protein Tyrosine Phosphatase(25 kD) purified using MagListo™ His-tagged Protein Purification Kit and two market leading competitors' products.
MagListo™ His-tagged Protein Purification Kit outperformed two competitors' similar products in terms of yield and purity.

Lane M: AccuLadder™ Protein Size Marker (Broad)
Lane 1: Crude extract
Lane 2, 5: Bioneer
Lane 3, 6: Company Q
Lane 4, 7: Company E
Washing: Samples taken after the washing step
Elution: eluted protein samples


figure2

Figure 2. Various sized proteins purified using MagListo™ His-tagged Protein Purification Kit.
Proteins as small as 10 kD and as large as 120 kD can be purified with the MagListo™ His-tagged Protein Purification Kit.

Lane M: AccuLadder™ Protein Size Marker (Broad)
Lane 1: TLA DNA Polymerase
Lane 2: PTPase #54
Lane 3: PTPase pk6


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Figure 3. Concentration comparison of TLA DNA polymerase proteins purified using MagListo™ His-tagged Protein Purification Kit and two market leading competitors' products.
MagListo™ His-tagged Protein Purification Kit showed up to 5-times higher concentration than that of the competitors.




Procedure

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