AccuLadder™ 3-color Prestained Protein size marker (Broad)

Features and Benefits

• Broad range

  Can identify proteins between 11 - 245 kDa when used with Tris-glycine-SDS running buffer.

• Ready-to-use

  Simply use the product directly as it is already supplied in a loading buffer; no additional process, such as boiling or adding buffer, is needed.

• Easy to identify

  Includes 25, 75 kDa reference bands coupled with a green and a red dye.

• Highly Reproducible

  Reproducible results with uniform quality products for each batch by manufacturing under the ISO 9001 quality system.

Application

• Monitoring of protein migration during SDS-PAGE.
• Monitoring of protein transfer onto membranes during Western blots.
• Sizing of proteins on SDS-PAGE and Western blots.

Technical Note

 Storage Buffer

Approximately 0.1 - 0.4 mg/ml of each proteins are in the buffer (20 mM Tris phosphate, pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.
 

 Storage condition

Can be stored maximum 3 months at 4°C, For long term storage, store at -20°C.
Please divide the products in small volumes and store them separately to avoid repetitive freezing and thawing.
 

 Quality control assay

Our SDS-Polyacrylamide gel anlaysis clearly shows distinguighable 12 bands.
 

 How to use

1. Thaw the ladder either at room temperature or at around 37 - 40°C for a few minutes to dissolve the precipitated solids (Do not boil).
2. Mix thoroughly to ensure homogeneity.
3. Load the following volumes of the ladder on the SDS-PAGE gel:
   • 5 μl/well for mini-gels, 2.5 μl/well for blots
   • 10 μl/well for large-gels, 5 μl/well for blots
4. Load the samples.
5. Connect the power supply and start the electrophoresis.
    

 Guide for Estimating Molecular Weight Estimation (kDa)

The migration patterns of AccuLadder™ 3-color Prestained Protein size marker (Broad) for different electrophoresis conditions can be viewed in the diagram below. In the prestained protein marker, the coupling of the protein chromophores is affected more in the apparent molecular weights in the SDS-PAGE compared to that of the unstained ones. Therefore, to achieve the accuracy of protein molecular weight measurements to be more than 95%, we recommend using the unstained protein markers.