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Ultrapure phosphoramidites from Bioneer

For DNA/RNA oligonucleotide synthesis, gene synthesis, and more

Overview

Oligonucleotide synthesis, siRNA and more

Bioneer has long been a leading manufacturer of phosphoramidites and oligonucleotides and has extensive expertise and experience in these areas.
Bioneer is an ISO 9001:2000-certified company with all products being manufactured under strict ISO 9001:2000 standards.


Reactions in Synthetic Reactors














Bioneer has four 6,000 L capacity reactors for synthesizing four different nucleosides. All nucleoside phosphoramidites are manufactured in 6,000 L reactors. Each reactor has a capacity of up to 50 kg per batch. Per annum total manufacturing capacity for nucleoside phosphoramidites is approximately 4 tons.





Purification of phosphoramidites















The resulting DNA nucleoside phosphoramidites are purified with column chromatography for purities of ≥99%. Four sets of 400 L volume columns are utilized for the separation of each different nucleoside phosphoramidite.







Filtering/Drying















The purified/crystallized nucleoside phosphoramidites are finally filtered and dried to yield the final product in powder form.
The process is carefully moisture-controlled to maintain less than 40 ppm water content after being dissolved in acetonitrile, the solvent for oligonucleotide synthesis.








All PRODUCTS: FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Products may be covered by pending or issued patents in certain countries.

©2010 Bioneer Corporation