For nucleic acid labeling, microarray labeling, and more

Features of labeling modifiers

• Oligonucleotide 5' end labeling can be used for fluorescent probe synthesis. Oligonucleotides modified with fluorescein derivatives can be used for genomic research, molecular diagnostics and molecular biology.
• Chemical phosphorylation reagents are most commonly used to phosphorylate the 5'-terminus of an oligonucleotide.
• 5'-Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5'-terminus during solid phase oligonucleotide synthesis. The DMT (dimethoxytrityl) group on the biotin moiety can be removed during synthesis to measure coupling efficiency or retained on the oligonucleotide to facilitate RP or cartridge purification. The 5'-biotin label is attached to the oligonucleotide through a 6-carbon atom (aminohexanol) spacer arm.
• DL-phosphoramidites can perform as universal bases as they hybridize with any of the four natural bases.
• 2'-F-RNA oligonucleotides adopt an A-form helix on hybridization to a target. Aptamers composed of 2'-F-RNA bind targets with higher affinity and are more resistant to nucleases compared to RNA aptamers. 2'-F-RNA can be effectively used in siRNA applications, especially in RNA interference for the specific silencing of genes in cells and in vivo. Bioneer provides three monomer types: 2’-F-rC(Bz) phosphoramidite, 2’-F-rC(Ac) phosphoramidite and 2’-F-rU phosphoramidite.
• 2'-OMe oligo ribonuleotides are extremely useful reagents for a variety of molecular biology applications. 2'-OMe-RNA is chemically more stable than either DNA or RNA and is resistant to degradation by RNA- or DNA- specific nucleases. Bioneer provides two monomers: 2'-OMe-rU-phosphoramidite and 2'-OMe-rC phosphoramidite.
• The Internal-dT amine phosphoramidite is a sequence modifier (amino-modifier) which can be set in the target position during the oligonucleotide synthesis process. After oligo synthesis and the deprotection process have been performed, the internal- dT amine can form a primary amine on the phosphoramidite. This primary amine can be bind with other target materials that have a NHS-ester moiety.
• Phosphoramidite modified with steroid derivatives can be used for improvement of hydrophobic interaction in cell, such as membrane organization. Cholesterol, the most common naturally occurring lipids in all existence, is a representative steroid derivative. Oligonucleotides modified with cholesterol derivatives can be used for genomic research and molecular biology.
• With an excellent stability profile, ferrocene has always attracted considerable interest for DNA labeling to generate probes for electrochemical detection.
• As a steroid with potent anti-inflammatory and immunosuppressive properties, β-methasone and cortisone are used as medicinal ingredients to treat many different conditions such as allergic disorders, skin conditions, ulcerative colitis, arthritis, lupus, psoriasis and breathing disorders.