AccuPower VTEC Taq PCR Kit
Specfications
| Instrument | AllInOneCycler™ Thermal Block or other Thermal Block |
|---|---|
| Specimen | Food, Environmental sample etc. |
| Time to result | about 2hr. 40min. |
| Tests per Kit | 96 |
Product Overview
AccuPower® VTEC Taq PCR Kit is designed to detect E.coli O157:H7 and VTEC simultaneously.
- Verotoxigenic E. coli, (VTEC) is a typical food poisoning-causing pathogenic Escherichia coli. It causes food poisoning such as abdominal pain, fever, nausea, vomiting, hemorrhagic colitis and hemolytic uremic syndrome when food contaminated with it is ingested.
- This test method detects both E. coli O157:H7 and verotoxigenic E. coli.
- Synonym : Shigatoxigenic Escherichia coli (STEC), Enterohemorrhage E. coli (EHEC), Vero-toxin producing E. coli(VTEC)
Features and Benefits
- Symplicity
Tracking dyes and sedimentation are included, so not necessary sample loading buffers when electrophoresis. Therefore, carry-over contamination by aerosol generation can be minimized. - Excellent reproducibility
Use of dried-type PCR PreMix only requires simple addition of template DNA and DW, which provides highly reproducible results. - Prevention of amplicon-induced cross-contamination by 8-MOP
Binding of 8-MOP (8-methoxypsoralen) to DNA double-helix structure, followed by UV irradiation. - High Stability
Two-year shelf life guaranteed at -20 °C. - Quality Control
All AccuPower® products are manufactured under ISO 9001 Quality Control System.
Figures
Figure 1. Performance test of AccuPower® VTEC Taq PCR kit(Veterinary)
Sensitivity tests were performed by diluting the positive control VT1 DNA and VT2 DNA in 10-fold increments (100 pg to 100 fg for samples 1 to 4). The reaction conditions were 95 °C for 60 sec. 65 °C 120 sec. 72 °C for 90 seconds (10 cycles), 95 °C for 60 seconds. 65 °C 120 sec. 72 °C for 90 seconds (10 cycles), 95 °C for 60 seconds. 64 °C -> 60 °C (1 °C / decrease) 120sec. 72 °C for 90 seconds (5 cycles), 95 °C for 60 seconds. 60 °C 120 sec. 72 °C for 90 seconds (10 cycles), 95 °C for 60 seconds. 60 °C 120 sec. The PCR reaction was carried out at 72 °C for 150 seconds (10 cycles) and 4 °C storage conditions. The 5 PCR reaction product was electrophoresed on 2% TBE agarose gel.
Line M : Marker
Line 1 : Vet_VT1 DNA 500pg + VT2 DNA 500pg
Line 2 : Vet_VT1 DNA 50pg + VT2 DNA 50pg
Line 3 : Vet_VT1 DNA 5pg + VT2 DNA 5pg
Line 4 : Vet_VT1 DNA 500fg + VT2 DNA 500fg
Line N : No template control
Sensitivity tests were performed by diluting the positive control VT1 DNA and VT2 DNA in 10-fold increments (100 pg to 100 fg for samples 1 to 4). The reaction conditions were 95 °C for 60 sec. 65 °C 120 sec. 72 °C for 90 seconds (10 cycles), 95 °C for 60 seconds. 65 °C 120 sec. 72 °C for 90 seconds (10 cycles), 95 °C for 60 seconds. 64 °C -> 60 °C (1 °C / decrease) 120sec. 72 °C for 90 seconds (5 cycles), 95 °C for 60 seconds. 60 °C 120 sec. 72 °C for 90 seconds (10 cycles), 95 °C for 60 seconds. 60 °C 120 sec. The PCR reaction was carried out at 72 °C for 150 seconds (10 cycles) and 4 °C storage conditions. The 5 PCR reaction product was electrophoresed on 2% TBE agarose gel.
Line M : Marker
Line 1 : Vet_VT1 DNA 500pg + VT2 DNA 500pg
Line 2 : Vet_VT1 DNA 50pg + VT2 DNA 50pg
Line 3 : Vet_VT1 DNA 5pg + VT2 DNA 5pg
Line 4 : Vet_VT1 DNA 500fg + VT2 DNA 500fg
Line N : No template control
Literature/Support
- Quality Assurance