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EcoQprep™ Genomic DNA Kit

 Catalog NumberDescriptionPrice(USD) 
♦K-3701EcoQprep™ Genomic DNA Kit$156.00add cart

Specfications

Components
TL Buffer15 mL(1 ea)
GB Buffer15 mL(1 ea)
WM1 Buffer30 mL(1 ea)
WB2 Buffer10 mL(1 ea)
WE Buffer40 mL(1 ea)
EA Buffer25 mL(1 ea)
Proteinase K powder, lyophilized25 mg(1 ea)
RNase A powder, lyophilized24 mg(1 ea)
Poly(A), lyophilized1 mg (1 ea)
Magnetic Nanobead6 mL (1 ea)
1.5 mL Tube50 ea(1 pack)
One Page Protocol1 ea
* Mini-50 rxn, Midi-7 rxn, Maxi-3 rxn
Shipping conditionroom temperature

Product Overview

EcoQprep™ Genomic DNA Kit is a magnetic beadbased system for isolating high-quality genomic DNA from cultured cells, animal tissues, and plant tissues. Using specially designed magnetic nanobeads and the EcoQprep™ Magnetic Separation Rack, the kit provides a faster and simpler workflow than conventional spin column methods. Because it does not require centrifugation, it reduces energy consumption and minimizes plastic waste. The purified genomic DNA is ready for downstream applications such as PCR, qPCR, sequencing, genotyping, and cloning.

Features

  • Efficient magnetic bead-based extraction
    Magnetic particles enable rapid nucleic acid separation with a magnet instead of centrifugation.

  • Eco-friendly nucleic acid extraction
    Reduces plastic waste compared to spin column filters.

  • Compatible with a wide range of sample types
    Extracts high-purity, high-yield genomic DNA from a wide range of samples, including whole blood, cultured cells, animal tissues, Gram-negative and Gram-positive bacteria, and forensic specimens.

  • Compatible with various scales
    Processes Mini, Midi, and Maxi scales with a single kit, allowing for flexible handling of sample volumes ranging from small to large.

  • Reliable extraction from small sample volumes
    Extracts DNA from as few as 1x104 cultured cells, making it ideal for processing small samples.

  • Fast processing time
    Using magnetic particle technology, nucleic acid extraction takes approximately 5 minutes* for Mini, 10 minutes* for Midi, and 15 minutes* for Maxi, significantly improving experimental efficiency.

  • Minimal equipment needed
    Only EcoQprep™ Magnetic Separation Rack(TM-1012) is required—no additional equipment needed.
*Excluding pre-treatment time

 

Applications

 

  • Gene cloning
  • PCR
  • Real-time PCR
  • Southern blotting
  • SNP genotyping
  • Short Tandem Repeat (STR) analysis

Specifications

Scale Micro Mini
 Midi
 Maxi
Whole blood Amount of Starting Sample 100 μL 200 μL 2 mL 4 mL
Typical DNA Yield [μg] < 5 μg < 10 μg < 80 μg < 150 μg
Cultured cells Amount of Starting Sample ~ 1 x 104 cells ~ 1 x 106 cells ~ 5 x 106 cells ~ 1 x 107 cells
Typical DNA Yield [μg] < 120 ng < 12 μg < 60 μg < 120 μg
Animal tissue Amount of Starting Sample ~ 10 mg ~ 25 mg ~ 100 mg ~ 250 mg
Typical DNA Yield [μg] < 5 μg < 10 μg < 40 μg < 120 μg
Bacteria
(Gram (-), (+))		 Amount of Starting Sample - ~ 1 x 109 cells ~ 5 x 109 cells ~ 1 x 1010 cells
Typical DNA Yield [μg] - < 15 μg < 80 μg < 150 μg
DNA purity A260/280 > 1.8
Turnaround Time > 5 min > 5 min > 10 min > 15 min

Various kinds of forensic sample
Amount of Starting Sample Dried body fluid spot or fingerprint
(FTA card, paper, cloth, etc)		 ~ 7 mm or ~ 2 cm2
Hair 1 cm
Bone and teeth ~ 100 mg
Chewing gum ~ 30 mg
Cigarette butts ~ 2 cm2
Buccal swab Single piece of swab
Urine ~ 15 mL
Saliva 1-100 μL
Typical DNA Yield [μg] ~ 5 μg
DNA purity A260/280 > 1.8
Turnaround Time > 10 min
    * Measurements may differ by sample type.

Figures


Figure 1. Comparison of Genomic DNA Extraction from Various Samples using EcoQprep™ Genomic DNA Kit and Competitors kit (column type).

(a) Whole blood: DNA extracted from 200 µL
(b) Animal tissue: DNA extracted from 15 mg of porcine skeletal muscle tissue
(c) HeLa cell: DNA extracted from 1x10 cells/rxn
(d) Escherichia coli : DNA extracted from 1x10 cells/rxn (DH5)

Figure 2. Genomic DNA Extraction from Various Samples using EcoQprep™ Genomic DNA Kit at Mini and Midi scales.

Figure 3. Comparison of real-time qPCR data of genomic DNA from HeLa cell (1 x 101 - 1 x 105) purified with EcoQprep™ Genomic DNA Kit and competitors kit (single column type).

(a) Amplication curve of GAPDH from HeLa cell (1 x 101 - 1 x 105) genomic DNA purified with Bioneer EcoQprep™ Genomic DNA Kit.
The premix used was Accupower® Plus DualStar™ qPCR PreMix (K-6600).
(b) Comparison of standard curves from qPCR results using genomic DNA purified with Bioneer EcoQprep™ DNA Kit and competitor A kit.

Figure 4. Genomic DNA Extraction from Paper Using EcoQprep™ Genomic DNA Kit Compared with a Competitors kit (column type).

After DNA extraction, real-time PCR is performed using the AccuPower® Plus DualStar™ qPCR PreMix with GAPDH as the target.
(a) Blood: DNA extracted from a 7 mm diameter punched sample of dried human whole blood (25 µL) on Whatman FTA card.
(b) Saliva: DNA extracted from a 7 mm diameter punched sample of dried human saliva (10 µL) on paper.
(c) Fingerprint: DNA extracted from a 7 mm diameter punched sample of fingerprint traces on paper.

Figure 5. Genomic DNA Extraction from Fabric Using EcoQprep™ Genomic DNA Kit Compared with a Competitors kit (column type).

After DNA extraction, real-time PCR is performed using the AccuPower® Plus DualStar™ qPCR PreMix with GAPDH as the target.
(a) Blood: DNA extracted from a 7 mm diameter punched samples of human blood (10 µL) stains on fabric.
(b) Saliva: DNA extracted from a 7 mm diameter punched samples of human saliva (10 µL) stains on fabric.

Figure 6. Genomic DNA Extraction Using EcoQprep™ Genomic DNA Kit Compared with a Competitors kit (column type).

After DNA extraction, real-time PCR is performed using the AccuPower® Plus DualStar™ qPCR PreMix with GAPDH as the target.
(a) Bone: DNA extraction from porcine bone 100 mg.
(b) Nail Clipping: DNA extraction from 15 mg of nail clipping.
(c) Hair: DNA extraction from 1 strand of hair with or without root.
(d) Cotton swab: DNA extraction from cotton swab with human saliva.
(e) Chewing Gum: DNA extraction from chewing gum with human saliva.

Literature/Support