EcoQprep™ mRNA Kit
| Catalog Number | Description | Price(USD) | ||
|---|---|---|---|---|
| ♦ | K-3708 | EcoQprep™ mRNA Kit | $399.00 |  |
Specfications
| Expected Total RNA Yield (mRNA typically accounts for 15% of total RN) | ~ 100 µg |
|---|---|
| Minimum Elution Volume | 15 µL |
| Extraction Time | ~ 10 min |
| Components (Mini-50 rxn) | |
| MRD Buffer | 30 mL(1 ea) |
| MRW1 Buffer | 40 mL(1 ea) |
| MRW2 Buffer | 50 mL(1 ea) |
| MRW3 Buffer | 50 mL(1 ea) |
| EM Buffer | 25 mL(1 ea) |
| Magnetic Nanobead-Oligo dT (10 mg/mL) | 3 mL (1 ea) |
| 1.5 mL Tube | 50 ea(1 pack) |
| One Page Protocol | 1 ea |
* Results may differ according to sample types.
Product Overview
EcoQprep™ mRNA Kit is a magnetic beadbased system for isolating high-quality mRNA with poly(A) tails from cultured cells, animal tissues, and plant tissues. Using oligo(dT) magnetic nanobeads and the EcoQprep™ Magnetic Separation Rack, the kit provides a faster and simpler workflow than traditional spin column methods. Because it does not require centrifugation, it reduces energy use and plastic waste. The purified mRNA is ready for downstream applications such as RT-PCR, RT-qPCR, cDNA synthesis, and gene cloning.
Features
-
High-purity, high-yield mRNA isolation
Oligo(dT) magnetic nanobeads selectively capture polyadenylated mRNA, efficiently removing genomic DNA and ribosomal RNA to deliver highly pure results. -
Eco-friendly nucleic acid extraction
Compared with conventional column filter methods, the magnetic bead–based workflow reduces plastic waste. - Broad sample compatibility
Enables high-quality mRNA extraction from diverse sources, including mammalian cells, animal tissues, and plant tissues. -
Rapid processing time
Magnetic bead technology allows mRNA extraction in approximately 10 minutes* - Minimal equipment required
Only theEcoQprep™ Magnetic Separation Rack(TM-1012) is needed—no centrifuge or additional instruments are required. -
Automation compatible
Fully compatible with the ExiPrep™ 96 Lite (A-5250, RUO) automated nucleic acid extraction system, enabling simultaneous processing of up to 96 samples.
*excluding sample pretreatment
Applications
- cDNA synthesis
- Reverse transcription PCR(RT-PCR)
- Reverse transcription quantitative PCR(RT-qPCR)
- Northern blot analysis
- RNA sequencing
Figures
Table 1. Yield and purity of total RNA and mRNA purified from various samples.
Figure 1: Total RNA was purified using the AccuPrep® Universal RNA Extraction Kit, and polyadenylated mRNA was purified using the EcoQprep™ mRNA Kit.
RNA yield (µg) and purity (A260/A280) were measured with a spectrophotometer. The mRNA ratio in total RNA (%) was calculated from the proportion of mRNA yield relative to total RNA yield. Samples tested included HeLa cells (2 10 cells), mouse liver (10 mg), mouse brain (10 mg), and broccoli (40 mg).
RNA yield (µg) and purity (A260/A280) were measured with a spectrophotometer. The mRNA ratio in total RNA (%) was calculated from the proportion of mRNA yield relative to total RNA yield. Samples tested included HeLa cells (2 10 cells), mouse liver (10 mg), mouse brain (10 mg), and broccoli (40 mg).
Figure 2. Comparison of real-time two-step RT-qPCR amplification of HPRT1 from HeLa cells using total RNA and mRNA purification kits.
HeLa cells (2 10 2 10 cells) were used as input samples. Total RNA was purified with the AccuPrep® Universal RNA Extraction Kit, and polyadenylated mRNA was purified with the EcoQprep™ mRNA Kit. Amplification of the HPRT1 gene was performed by two-step RT-qPCR.
[Total RNA] Amplification curves obtained from total RNA.
[mRNA] Amplification curves obtained from mRNA.
[Table] Ct values of HPRT1 detected from serial dilutions of HeLa cells. UD = undetermined.
HeLa cells (2 10 2 10 cells) were used as input samples. Total RNA was purified with the AccuPrep® Universal RNA Extraction Kit, and polyadenylated mRNA was purified with the EcoQprep™ mRNA Kit. Amplification of the HPRT1 gene was performed by two-step RT-qPCR.
[Total RNA] Amplification curves obtained from total RNA.
[mRNA] Amplification curves obtained from mRNA.
[Table] Ct values of HPRT1 detected from serial dilutions of HeLa cells. UD = undetermined.