ExiPrep™ Tissue Genomic DNA Kit (96 reactions)
| Catalog Number | Description | Price(USD) | ||
|---|---|---|---|---|
| ♦ | K-3225 | ExiPrep™ Tissue Genomic DNA Kit (96 reactions) | $381.15 |  |
Specfications
| Specifications | |
|---|---|
| Starting culture volume | up to 25 mg |
| Elution volume | 50 - 100 µl |
| Expected yield | 5 - 20 µg |
| Expected purity | A260/280: > 1.8 |
| Components | |
| Buffer Cartridge (1) | 6 ea |
| Buffer Cartridge (2) | 6 ea |
| Tissue lysis buffer | 1 ea(25 ml) |
| Disposable filter tip | 3 pack (32 ea/pack) |
| Reaction tube | 1 pack (96 ea/pack) |
| Elution tube | 1 pack (12 ea/pack) |
| User's Guide | 1 ea |
Product Overview
ExiPrep™ Tissue Genomic DNA Kit allows rapid extraction of highly pure genomic DNA with high yield from animal tissue samples using automatic nucleic acid extraction instruments: ExiPrep™16 series (ExiPrep™16, ExiPrep™16 Plus) and ExiProgen™. Pre-filled buffer cartridges with essential reagents for nucleic acid extraction can be used to isolate maximum of 16 samples simultaneously with ease. Furthermore, following the preparation method in the user manual, the Tissue Lysis Buffer and Proteinase K included in this product can effectively break down animal tissues.
Features and Benefits
-
Pre-filled buffer cartidge system
Diluted enzymes and reagents essential for nucleic acid extraction -
Great lysis optimization
Tissue Lysis Buffer and consumables (Disposable filter tip, Elution tube, etc.) included in the kit for various animal tissue lysis -
Quick purification time
Simultaneous processing of at least 1 to 16 large samples at maximum -
High-quality, high-yield quick productivity
Implementation of Silica Magnetic Particles developed and produced by Bioneer's proprietary technology -
High reproducibility
Reliable results through the use of automatic nucleic acid extraction instrument (ExiPrep™ 16, ExiPrep™ 16 Plus, ExiProgen™)
Application
- PCR
- Quantitative real-time PCR
- Gene cloning
- SNP analysis
- Genetics
Procedure
Figures
Figure 1. Agarose gel electrophoresis results of genomic DNA extracted from mouse tissue
Results of electrophoresis of genomic DNA extracted from mouse tissue cells (lung, 25 mg) on 1% agarose gel were shown above. To check the occurrence of cross-contamination during genomic DNA extraction, mouse tissue samples were placed sparsely in a checkerboard pattern while the rest wells were filled with distilled water (D.W.). No signs of cross-contamination were found in the extracted genomic DNA, with the average yield of 20 µg and the mean purity (A260/280)of at least 1.8.
- M; Size marker
- S; Extraction with mouse tissue sample
- B; Extraction with D.W. only.
- M; Size marker
- S; Extraction with mouse tissue sample
- B; Extraction with D.W. only.
Results of electrophoresis of genomic DNA extracted from a mouse tail tip (~ 1 cm) on 1% agarose gel were shown above. To check the occurrence of cross-contamination during genomic DNA extraction, mouse tail-tip samples were placed sparsely in a checkerboard pattern while the rest wells were filled with distilled water (D.W.). No signs of cross-contamination were found in the extracted genomic DNA, with the average yield of 20 µg and the mean purity (A260/280) of at least 1.8.
- M; Size marker
- S; Extraction with mouse tail-tip sample
- B; Extraction with D.W. only.
- M; Size marker
- S; Extraction with mouse tail-tip sample
- B; Extraction with D.W. only.
Literature/Support
- Brochure
- Manual
- Quality Assurance