Rocketscript™ Reverse Transcriptase
Specfications
| Specifications | |
|---|---|
| 5' to 3' exonuclease | No |
| 3' to 5' exonuclease | No |
| 3' A overhang | No |
| Fragment size | Up to 10 kb |
| Components(E-3141) | |
| RocketScript Reverse Transcriptase | 50 ul |
| RNase Inhibitor(100ng/ul) | 50 ul |
| 5X Reaction buffer | 0.5 ml |
| 10 mM dNTPs | 0.2 ml |
| 100 mM DTT | 0.3 ml |
| Etc | |
| Concentration | 10,000 Units (200 U/µl) |
- 5x Reaction Buffer: Tris-HCl, KCl, MgCl2, Stabilizers (pH8.5)
- 100 mM DTT
- 10 mM dNTPs mix: 2.5 mM of each dNTP
| Shipping condition | dry ice |
|---|---|
| Storage condition | -20°C, 50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, Stabilizers. |
Product Overview
RocketScriptTM Reverse Transcriptase is an independently developed thermostable reverse transcriptase derived from M-MLV. Its resistnace to heat (42 ~ 70°C) allows effective synthesis of complex RNA structures with full-length cDNA.
Features and Benefits
- Thermostablity
Performance at comprehensive temperature conditions from 42 to 70 °C for cDNA synthesis with Rocketscript ™ series overcoming the limitation of traditional 42°C RT reactions
- User-friendly
Simplified procedure by including all components necessary for cDNA synthesis such as Thermostable Reverse Transcriptase, RNase inhibitor, etc.
- Reproducibility
Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system
Application
- Gene synthesis
- First-strand synthesis of cDNA from RNA molecules (reverse transcription)
- RT-PCR
- Random priming reactions
- Library construction
- Probe labeling
- mRNA 5'-end mapping by primer extension analysis
- Real time PCR
Figures
Figure 1. Complex RNA amplification.
Complex RNA amplification results of RocketScript™ Reverse Transcriptase compared with that of conventional RTase.
Reverse transcription condition: conventional 42°C/50°C/60°C 1 hr, deactivation 95°C 5 min, This product shows thermal stability.
Lane A; M-MLV Reverse Transcriptase
Lane B; RocketScript™ Reverse Transcriptase
Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell
Complex RNA amplification results of RocketScript™ Reverse Transcriptase compared with that of conventional RTase.
Reverse transcription condition: conventional 42°C/50°C/60°C 1 hr, deactivation 95°C 5 min, This product shows thermal stability.
Lane A; M-MLV Reverse Transcriptase
Lane B; RocketScript™ Reverse Transcriptase
Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell
Figure 2. Thermostable Stability Check.
Amplification results of RocketScript™ Reverse Transcriptase using myc compared with supplier's reverse transcription condition: incubation at each temperature 45/50/55/60/65/70 °C for 1 hr, deactivation at 95°C for 5 min
Primer set: human myc 495 bp set
Lane M; 1 kb DNA Ladder(Bioneer, Cat. No. D-1040)
Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell
Lane 3; 1 ng human total RNA from HeLa cell
Lane 4; 100 pg human total RNA from HeLa cell
Amplification results of RocketScript™ Reverse Transcriptase using myc compared with supplier's reverse transcription condition: incubation at each temperature 45/50/55/60/65/70 °C for 1 hr, deactivation at 95°C for 5 min
Primer set: human myc 495 bp set
Lane M; 1 kb DNA Ladder(Bioneer, Cat. No. D-1040)
Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell
Lane 3; 1 ng human total RNA from HeLa cell
Lane 4; 100 pg human total RNA from HeLa cell
Figure 3. Comparison of amplification quality between RocketScript™ Reverse Transcriptase and competitor Reverse Transcriptase.
Sensitivity test. Target gene expression Level.
Lane M; 1 kb DNA Ladder
Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell
Lane 3; 1 ng human total RNA from HeLa cell
Lane 4; 100 pg human total RNA from HeLa cell
Sensitivity test. Target gene expression Level.
Lane M; 1 kb DNA Ladder
Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell
Lane 3; 1 ng human total RNA from HeLa cell
Lane 4; 100 pg human total RNA from HeLa cell