Bioneer’s AccuPrep 96 PCR Purification Kit is a high throughput PCR purification kit that allows PCR product
purification from up to 96 PCR reactions at once. The AccuPrep 96 PCR Purification Kit allows purification of
up 10 μg of DNA fragments from PCR and other enzymatic products within 25 minutes. The AccuPrep 96 PCR
Purification Kit features recovery efficiencies up to 95%. Size range is 100 bp - 10 kb, thus 20-40 mer
oligonucleotides and primer dimers found in PCR reactions are easily removed. Removal of mineral oil is
The principle of the AccuPrep 96 PCR Purification Kit is based on the adsorption of DNA in glass filter.
Purification is accomplished with the BioVac 96 Vacuum Manifold. Chaotropic salt enhances the binding of
DNA onto a glass filter that is fixed in the AccuPrep 96 Binding plate. Adsorption of DNA is very selective
so that other proteins and salts are not adsorbed and pass through the glass filter. Washing eliminates salts
and other proteins that may bind non-specifically. High-purity DNA fragments are eluted with provided
AccuPrep EL Buffer (10 mM Tris-Cl, pH 8.5) or distilled water. Purified DNA fragment can be immediately
used for subcloning, sequencing and other molecular biological applications.
Features and Benefits
||Ready-to-use DNA in 25 minutes
||Reproducibly high yields of molecular biology grade DNA
||Great value for your dollar