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AccuPower® Rocketscript ™ RT-PCR PreMix & Master Mix

 Catalog NumberDescriptionPrice(USD) 
 K-2501AccuPower® RocketScript™ RT-PCR PreMix, 0.2 ml 8-tube strip, 20 µl x 96 rxn$325.20add cart
 K-2503AccuPower® RocketScript™ RT-PCR PreMix, 0.2 ml 8-tube strip, 50 µl x 96 rxn$484.80add cart
♦K-2502AccuPower® RocketScript™ RT-PCR PreMix, 0.2 ml 8-tube strip, 20 µl x 480 rxn$1219.20add cart
 K-2504AccuPower® RocketScript™ RT-PCR PreMix, 0.2 ml 8-tube strip, 50 µl x 480 rxn$1801.20add cart
 K-2505AccuPower® RocketScript™ RT-PCR Master Mix (2X, 1ml)$304.25add cart

Specfications

5' to 3' exonucleaseNo
3' to 5' exonucleaseNo
3' A OverhangYes
Fragment SizeUp to 6 kb
Shipping conditiondry ice

Product Overview

AccuPower® RocketScript™ RT-PCR PreMix is a one-step RT-PCR product that uses Thermostable RTase (RocketScript™ Reverse Transcriptase) and vacuum-dried ProFi Taq DNA polymerase, dNTP, reaction buffer, etc. By using the RocketScript™ Reverse Transcriptase, RT reactions can be performed at a higher temperature for obtaining template RNA with strongly bounded secondary structure. Not only it has high elongation and sensitivity, but also contains all the components necessary for cDNA synthesis and electrophoresis, including the tracking dyes, to perform RT-PCR reaction directly just by adding the RNA, primer, and D.W.

Features and Benefits

  • Long kb RT-PCR

    Synthesis of full-length size of 5.2 kb (target: human TFRC) target through one-step RT-PCR using human total RNA 100 ng

  • Sensitivity

    Synthesis using only 10 pg of human total RNA pg (target: ß-Actin) extracted from cells and tissues through one-step RT-PCR

  • Thermostable Activity

    Excellent thermal resistance of RocketScript ™ RTase active even at 70°C, making it suitable for cDNA synthesis of secondary RNA structure with comprehensive selection of compatible temperature ranging from 42 to 70 °C depending on the required experimental condition and effective cDNA synthesis 

    overivew

    Note: Schematic representation of the 5'UTR of a gene, with complex secondary structure, at three different temperatures. Note that RocketScript™ shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other reverse transcriptase's fail. 

  • Reproducibility

    Mass produced in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product to provide reproducible experimental results

  • User-friendly

    All necessary components for cDNA synthesis such as thermostable reverse transcriptase, RNase inhibitor, etc., included to perform the reaction immediately just by adding the template RNA to be amplified, primer, and D.W.
    Tracking dye and sedimentation agents also included in the products for electrophoresis, making the loading buffer unnecessary to be added 

Application

  • First-strand synthesis of cDNA from RNA molecules (RT)
  • RT-PCR
  • cDNA library construction
  • Gene expression analysis

Figures


Figure. Performance comparison between AccuPower® RocketScript ™ RT-PCR PreMix and competitor RT-PCR kits
Complex secondary structure RNA species amplification by RocketScript ™ is outstanding compared to the leading competitor's reverse transcriptase. RT reactions were performed according to each manufacturer's recommendations.

Lanes 1-4 are 10 ng, 1 ng, 0.1 ng and 0.01 ng of total RNA from HeLa cells, respectively

Figure 1. AccuPower® RocketScript ™ RT-PCR PreMix shows enhanced performance compared to competitors.
One-step RT-PCR reactions were performed with 100 ng total RNA from HeLa cells using reagents and conditions specified in each manufacturer's protocol.

Lane 1; 3 kb
Lane 2; 4.5 kb
Lane 3; 5.2 kb

Figure 2. Highly reproducible Amplifications
Amplification of an 500 bp target gene was detected using human total RNA (from 10 ng to 10 pg) with AccuPower® RocketScript™ RT-PCR PreMix. As shown in Fig3, highly reproducible amplifications were achieved within each Lot. set of triplicates Lanes 1-4; 10 ng, 1 ng ,and 100 pg, 10 pg of total RNA from HeLa cells, respectively.

Literature/Support