AccuPower® HotStart PCR PreMix is a PCR master mix containing a thermostable DNA polymerase, thermostable
pyrophosphatase, reaction buffer, dNTPs, tracking dye, and patented stabilizer in a ready to use hotstart
PCR master mix.
Bioneer uses a unique enzyme-mediated HotStart PCR that provides robust and reliable results. Bioneer’s Top
DNA Polymerase is inhibited at lower temperatures (< 70°C) by pyrophosphate. However, Top DNA Polymerase is
rendered fully active at temperatures above 70°C via pyrophosphate hydrolysis with our thermostable
pyrophosphatase. This prevents the formation of mis-primed products and primer-dimers during the reaction set up
process resulting in improved PCR specificity. It is ideal for nucleic acid amplification reactions involving
complex genomic or cDNA templates, very low copy targets, and multiplex reactions.
Features and Benefits
| Ease of use: |
Just add template and primers and start your PCR, dNTPs, buffer and enzyme are provided |
| Stability: |
Stable at room temperature for a month and for 2 years in a -20°C freezer |
| HotStart: |
Unique enzyme mediated HotStart results in greater specificity and more robust reactions |