The AccuPower® DNA Ligation PreMix is a lyophilized master mix containing T4 DNA Ligase,
ATP, reaction buffer, and patented stabilizer. This DNA ligation premix is conveniently aliquoted in strip-tubes
for reactions; you need only add DNAs to be ligated and water. The reaction will work for DNA ligation for all
applications: blunt cloning, sticky end cloning and TA cloning. The premix is stable up to four months at room
temperature and for three years at -20°C.
Features and Benefits
| Ready to use premix: |
Minimal set up time and handling required |
| Fast: |
Only 5 minutes for cohesive-end ligation and 10 minutes for blunt-end ligation at room temperature |
| Stable: |
Enzyme activity for up to four months at room temperature and for three years in the freezer |