Exicycler™ V5

Features and Benefits

• Fast Ramping Rate & Scan time
  • Maximum ramping rate of 10.0°C/sec by implementing *nano metal alloy block. (* patent pending)
• 6-Multiplex PCR
  • 6 target genes can be detected in a single tube which prevents contamination & human error
  • Improved cost-effectiveness with less consumed reagents
• Stable light source and longer lifespan
  • Long shelf life and minimum calibration by using LED Light Source
• Advanced Performance
  • Reliable PCR results with improved temperature uniformity and accuracy
  • Supports a wide range of research including various applicapable qPCR kits and services from Bioneer
• Easy to use
  • Fast and convenient touch screen
  • Operate protocol and graph in real-time without PC
• Convenient features
  • Cycle Add function: PCR Cycles can be added during PCR Protocol operation
  • Open Data File function: Ability to view graphs by opening a previous data file

Excellent Accuracy and Uniformity

Excellent homogeneity of amplification

By implementing in-house designed LT technology in the optics module, well-to-well signal variation has been dramatically minimized and well-to-well optical homogeneity has been improved.
Total volume, Ct average, Ct max-min, SD and CV: 20µl, 19.48, 0.24, 0.06, 0.30%. No matter which well the reaction is executed, the Ct variation will be within 0.3.

Figure 1. qPCR result using 107 copies of Human HPRT1 gene in each of 96 well positions

Wide Linear Dynamic Range

The dynamic range of detection is wide at over 10-logs

ExiCycler™ V5 has 10 orders of magnitude wide dynamic range especially for the quantitative experiment.
Fluorescence data from a series of 10-fold dilution of IRF3 gene(1011 copies) amplified using reporter dyes to check on target: TexasRed/IRF3. Graph shows eleven Ct values about each dilution: 7.26, 11.04, 14.26, 17.46, 20.89, 24.15, 27.58, 31.16, 34.32,37.45, 40.26.

Figure 2. Graph shows standard curve of 10 fold serial dilutions of 10 copies to 1011 copies IRF3 gene (TexasRed labeled).
The PCR efficiency generated by the standard curve is 100%.

Precise target discrimination (1.33-fold serial dilutions)

Precise detection at a concentration difference

ExiCycler™ V5 is able to distinguish precisely from the sensitive concentration difference of target to be amplified. Fluorescence data from a series of 1.33-fold dilution of TMV DNA(106 copies) amplified using reporter dyes to check one target: TAMRA/TMV. Graph shows seven Ct values about each dilution: 23.2, 23.66, 24.25, 24.91, 25.36, 25.94, 26.51.

Figure 3. ExiCycler™ V5 provide sensitive detection and precise target discrimination down to 1.33-fold differences

Reliable results in a wide range of PCR volume

Precise temperature control system with cutting-edged algorithm provides reliable results in a wide range of PCR volume

The purpose of this test is to vary the PCR reaction volume from 5 to 50 ul and check the results according to the volume. Even with a small amount of PCR reaction of 5ul, the CV(%) value showed stable results at 0.58%, but ExiCycler™ V5 recommends the use in the range of 10~50ul for more reliable results, and 20ul is recommended as the optimal volume.Total volume, Ct average, SD and CV: 50µl, 21.49, 0.05, 0.23%, 30µl, 21.33, 0.07, 0.33%, 20µl, 0.03, 0.14%, 10µl, 21.14. 0.06, 0.28%, 5µl, 20.86, 0.12, 0.58%. At the recommended PCR volume of 20ul, the CV value was found to have the least variation at 0.14%.

Figure 4 . PCR amplification was performed at each volume using lambda DNA (FAM labeled) at a concentration of 107 copies.

Thermal Gradient function for optimizing annealing temperature

Using the thermal gradient function, users can easily find optimal experimental conditions at once.

By using the thermal gradient function, you can save time and consumables by eliminating the need for multiple unnecessary experiments. Human PGK1 gene(FAM labeled) was diluted to a concentration of 103 copies to 106 copies were amplified under temperature conditions of 50-64 degrees using the annealing gradient function. As a result of PCR efficiency measurement using the standard curve at each point, the annealing temperature was optimal at 56 degrees.

Figure 5. This graph shows the difference in PCR efficiency according to annealing temperature by experimenting with the thermal gradient function to optimize the annealing temperature.

Superior Sensitive Optics by Light Polarization

Bioneers imaging technique based on polarization of light provides sensitive detection for robust and reliable results.
(Patents: KR 1089045, US 8427643)

Patented polarizing optical apparatus mitigates the common problem of a reflection light, allowing precise quantification and target discrimination.

6-Target Multipexing PCR

Up to 6 target genes can be detected in a single reaction well.

ExiCycler™ V5 allows for a wider range of detection chemistries and assay multiplexing than previous models. There are six excitation filters (430-630 nm) and six emission filters (480-690 nm). A total of 10 types of fluorescent dyes including ATTO425, FAM / SYBR Green, TET/JOE, TAMRA/Cy3, Texas Red/ROX, and Cy5 have been calibrated, so the dyes can be used immediately.

* Spectrum of 6-Multiplex Fluresent dye

*Filter information Filter Excitation (nm) Emission (nm) Fluorescent dye F0 430 480 ATTO425 F1 470 520 FAM, SYBR® Geen I F2 515 550 JOE, TET F3 550 585 TAMRA, Cyanine3 F4 570 615 Texas Red®, ROX F5 630 690 Cyanine5

Figure 7. This graph shows the detection of 6 target genes in single reaction well with the ExiCycler™ V5

Intuitive & Convenient Software

It can be operated independently without a PC, and operation is simple with an easy-to-use touch screen.

Researchers can conveniently operate the instrument with simple operations and observe the amplification process in real-time. Additionally, the Open Data file function, a feature that competitors do not have, allows you to check the PCR graph by opening previous data after completing the experiment.

Run SW - Experiment information setup & graph