AccuLadder™ 3-color Prestained Protein size marker (Broad) allows to estimate the sizes of proteins during protein electrophoresis. This product is composed of 12 different types of proteins (11 - 245 kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively), allowing each band to be distinguished with ease. AccuLadder™ 3-color Prestained protein size marker (Broad) is designed for monitoring protein isolation when observing Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and estimating the sizes of proteins. The ladder is supplied in a gel loading buffer, making it ready to be used.

Features and Benefits

• Broad range :
  Can identify proteins between 11 - 245 kDa when used with Tris-glycine-SDS running buffer.
• Ready-to-use :
  Simply use the product directly as it is already supplied in a loading buffer; no additional process, such as boiling or adding buffer, is needed.
• Easy to identify :
  Includes 25, 75 kDa reference bands coupled with a green and a red dye.
• Highly Reproducible :
  Reproducible results with uniform quality products for each batch by manufacturing under the ISO 9001 quality system.

Application

• Monitoring of protein migration during SDS-PAGE.
• Monitoring of protein transfer onto membranes during Western blots.
• Sizing of proteins on SDS-PAGE and Western blots.

FAQ

• Can I use this product with protein less than 10 kDa? :
  
  • AccuLadder™ 3-color Prestained Protein size marker (Broad) (Cat. No. D-2030) can identifiy proteins between 11 and 245 kDa.
  • AccuLadder™ Protein size marker (Broad) (Cat. No. D-2010) is a unstained protein size marker that can be used to identify proteins between 6.5 and 116 kDa. AccuLadder™ Protein size marker (Low) (Cat. No. D-2020) product can be used to identify between 6.5 and 66 kDa.
  • For accurate results, we recommend using a mass spectrometer for small proteins or peptides.
• What sholud I do beforehand before using this product? :
  This product can be used immediately without any additional procedures. You do not need to add any loading buffers or undergo heat treatment.
• My protein bands are not clear on SDS-PAGE Gel. :
  We recommend to use 5 ul of the solution per well with mini gel having an area of 10x8(cm2) and thickness of 0.75 or 1.0 mm
  If the bands still show low sensitivity, of if a midi-gel or a large-gel is used, try increasing the loading amount of the product.
• Can I use this product for Coomassie Blue of Silver staining? :
  All our AccuLadder™ Protein size marker (Cat. No. D-2010, D-2020, and D-2030) are compatible with those staining.