For routine applications, Bioneer offers standard custom oligos with no modification, Bio-RP purification,
provided in a tube format. The chart in the ordering information tab shows pricing at various synthesis scales,
but please contact your sales representative to learn about any institutional or volume discounts we might have
in place for our oligonucleotides.
Features and Benefits
| Free Bio-RP purification: |
Near HPLC purity at a standard oligo price |
| Broad range of modifications available: |
If you don’t see it, just ask, we can probably do it |
| Competitive pricing: |
Great value for your research dollar |
Why is Bio-RP purification better than desalting?
Most oligos sold are merely deprotected/desalted. The process of deprotection/desalting only partially purifies the
oligo and leaves behind many impurities* and failure products (truncated oligos that will not amplify, and can even
interfere with certain applications). These can lead to artificially inflated OD readings that increase with oligo
length. Bio-RP purification removes all these contaminants and failure products, resulting in an oligo that is near
HPLC purity. The advantage to you is that our product contains only quality oligos without impurities that can
inhibit some PCR reactions, and potentially skew qPCR reactions. We have the same amount of active oligo as our
competitors; they just have more “other” inflating their OD. To demonstrate this, plates of Oligos of various sizes
were tested for concentration in 2 steps: 1) After a deprotection/desalting step only, and 2) after Bio-RP
purification. (Fig. 1)
The results of these tests show that the amount of failed sequences and impurities* increase with length (as expected),
but are not removed by deprotection/desalting alone. This results in inflated OD readings for oligos depending on oligo
size. (Table 1)
Figure 1. Plates of Oligos of various lengths were tested for concentration after desalting and then by Bio-RP
cartridge purification. Note inflated OD readings when deprotection/desalting alone is used.
| Oligo Size |
18 - 24 |
24 - 29 |
30 - 34 |
35 - 41 |
| OD Inflation |
38% |
45% |
60% |
77% |
Table 1. Average OD inflation seen for oligos of various sizes when protection/desalting alone is employed.
Note for longer oligos, up to 77% of product does not consist of functional oligo.
*Impurities in desalted oligos may include: Acetonitrile, Pyridine, Iodine, Ethylthio tetrazole, Dichloromethane,
Acetic acid, Acrylonitrile, Benzamide and Isobutyramide.