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Guaranteed Minimum Yield from Bioneer

Checked using MALDI-TOF or Sequenom MassARRAY oligo check

Overview
Literature/Support

Yield will vary depending on base composition, purification method, oligo length, and oligo synthesis scale. To better serve our customers, Bioneer has set standards for guaranteed yields. In addition, our Quality Control (QC) department checks each and every oligo that Bioneer ships out by MALDI-TOF or Sequenom MassARRAY Oligo Check. QC data is included with every shipment.

* Please note OD values apply to tube oligos, and nmol values apply to plate oligonucleotides.
* Please note the yield of oligos with modifications and PAGE or HPLC purification will be decreased by 15 to 20%.


Purification Level Oligo Length Unit 0.025 μmol 0.05 μmol 0.2 μmol 1.0 μmol 10 μmol 15 μmol
BIO-RP(Cartridge) 20mer nmol 9 19 37 134 1333 2005
OD 2 4 8 29 288 433
30mer nmol 6 12 25 90 892 1341
OD 2 4 8 29 288 433
40mer nmol 4 9 19 67 667 1002
OD 1.5 4 8 29 288 433
50mer nmol 3 7 15 54 534 803
OD 1.5 4 8 29 288 433
60mer nmol 2 6 12 45 444 668
OD 1 4 8 29 288 433
70mer nmol 5 11 38 382 574
OD 4 8 29 288 433
BioRP + Modification 20mer nmol 7 15 30 107 1066 1604
OD 2 3 6 23 230 346
30mer nmol 5 10 20 72 714 1073
OD 2 3 6 23 230 346
40mer nmol 3 7 15 54 534 802
OD 1.5 3 6 23 230 346
50mer nmol 2 6 12 43 427 642
OD 1.5 3 6 23 230 346
60mer nmol 1 5 10 36 355 534
OD 1 3 6 23 230 346
70mer nmol 4 9 30 306 459
OD 3 6 23 230 346
PAGE 20mer nmol 3.0 4 19 153 231
OD 0.7 0.9 4 33 50
30mer nmol 2.0 3 12 102 155
OD 0.7 0.9 4 33 50
40mer nmol 1.0 2 9 76 116
OD 0.7 0.9 4 33 50
50mer nmol 0.7 1 6 50 74
OD 0.5 0.8 3 27 40
60mer nmol 0.7 1 3 34 49
OD 0.5 0.8 2 22 32
70mer nmol 0.7 1 3 23 33
OD 0.5 0.8 2 17 25
HPLC 20mer nmol 3.0 4 28 185 277
OD 0.8 1 6 40 60
30mer nmol 2.0 3 18 123 186
OD 0.8 1 6 40 60
40mer nmol 1.0 2 13 92 140
OD 0.8 1 6 40 60
50mer nmol 0.7 1 6 65 92
OD 0.8 1 3 35 50
60mer nmol 0.7 1 4 46 61
OD 0.8 1 3 30 40
70mer nmol 0.7 1 3 33 40
OD 0.8 1 3 25 30
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