Custom Options (Master Mix, Reverse Transcriptase)

 

AccuPower® Dual-HotStart™ RT-qPCR Master Mix




AccuPower® Dual-HotStart™ RT-qPCR Master Mix is a one-step RT-PCR product that applied Pyro-Hotstart RT technology and Hotstart PCR technology to substantially improve the problems of non-specific reverse reaction and enhance the sensitivity to effectively undergo reverse transcription of template RNA even if it is present in a miniscule amount. Moreover, this product is compatible with comprehensive types of devices, including Exicycler™ 96 and those from other companies, by providing optimized tube and plates, along with a 2X master mix type.


  • Features and Benefits:
    • Specificity
      The world's first Dual-HotStart™ RT-qPCR reaction using PyroHotStart RT reaction and HotStart PCR optimized to detect only the target gene precisely.
    • Sensitivity
      Detection of miniscule amounts of the target templates in a high concentration of RNA samples unable to be done with convientional methods with a wide dynamic range of 10 logs up to 10 ~ 1010 copies.
    • Multiplex
      Compatible with many kinds of fluorescent dye (probes) to detect various kinds of target genes.
    • Comprehensive template RNA detection
      RocketScript ™ RTase included for undergoing RT reaction at high temperature and gaining RT-qPCR results from strong secondary template RNA structures


Catalog# Product
K-6707 2.5 ml of 2X Master mix solution




 

RocketScript™ Reverse Transcriptase




RocketScript™ Reverse Transcriptase is an independently developed thermostable reverse transcriptase derived from M-MLV. Its resistnace to heat (42 ~ 70°C) allows effective synthesis of complex RNA structures with full-length cDNA.
  • Features and Benefits:
    • Thermostablity
      Performance at comprehensive temperature conditions from 42 to 70 °C for cDNA synthesis with Rocketscript™ series overcoming the limitation of traditional 42°C RT reactions
    • User-friendly
      Simplified procedure by including all components necessary for cDNA synthesis such as Thermostable Reverse Transcriptase, RNase inhibitor, etc.
    • Reproducibility
      Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system


Catalog# Product
E-3141 Rocketscript™ Reverse Transcriptase 10,000 U (50 rxn)