For ligation of DNA, TA cloning, and other recombinant DNA applications

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will ligate DNA from blunt-end and cohesive-end termini (Including sticky ends for TA Cloning) as well as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids. T4 DNA Ligase is isolated from a recombinant E.coli strain.

Features and Benefits

High Speed:	DNA ligations in 5 minutes for cohesive end and in 10 minutes for blunt end DNA at 25°C
Flexibility:	Suitable for all common DNA ligations
Reproducibility:	Batch manufacturing under strict ISO 9001 quality control system