Specifications
5' to 3' exonuclease activity: No
3' to 5' exonuclease activity: No
Terminal transferase activity: Yes
Fragment Size: Up to 10 kb
Application
Standard PCR
Figure 1. High sensitivity of AccuPower® PCR PreMix
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Lane 1 : Lamda DNA 10ng
Lane 2 : Lamda DNA 1ng
Lane 3 : Lamda DNA 100pg
Lane 4 : Lamda DNA 10pg
Lane 5 : Lamda DNA 1pg
Lane 6 : Lamda DNA 100fg
Lane 7 : Lamda DNA 10fg
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Lane 8 : Lamda DNA 1fg
Lane 9 : Human genomic DNA 10ng
Lane 10 : Human genomic DNA 1ng
Lane 11 : Human genomic DNA 100pg
Lane 12 : Human genomic DNA 10pg
M: 100 bp plus DNA ladder (Bioneer Cat. No: D-1035)
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Figure 2. Comparison of heat-stability of AccuPower® PCR PreMix
AccuPower® PCR PreMix is incubated at 95℃ with various time.
Kit: AccuPower® PCR PreMix (Batch-1/2)
A : 30min, B : 60min, C : 90min
Lane 1 : Human genomic DNA 10ng
Lane 2 : Human genomic DNA 1ng
Lane 3 : Human genomic DNA 100pg
Lane 4 : Human genomic DNA 10pg
M: 100 bp plus DNA ladder (Bioneer Cat. No: D-1035)
Figure 3. Comparison of PCR amplification sensitivity between AccuPower® PCR PreMix from Bioneer and other suppliers‘ PCR kit.
Lane 1 : Lamda DNA 10ng
Lane 2 : Lamda DNA 1ng
Lane 3 : Lamda DNA 100pg
Lane 4 : Lamda DNA 10pg
Lane 5 : Lamda DNA 1pg
Lane 6 : Lamda DNA 100fg
Lane 7 : Lamda DNA 10fg
Lane 8 : Lamda DNA 1fg
Figure 4. Comparison of PCR amplification of long targets between AccuPower® PCR PreMix from Bioneer and other suppliers' PCR kit.
A: 5kb B: 6kb C: 7kb