AccuPower® PCR PreMix(with UDG) is a ready-to-use master mix for high-specificity DNA amplification, con-taining UDG to prevent carryover contamination or crossover contamination

 

Specifications

 

5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: Yes
Fragment Size: ~ 10 kb

 

Application

 

Polymerase chin reaction (PCR)
Primer extension
TA cloning
Gene sequencing
Molecular Diagnosis

 

Experimental data

 

 Sensitivity

Figure 1. Comparison of sensitivity between Accupower® PCR PreMix and Accupower® PCR PreMix(with UDG).
Sensitivity test was operated using serial diluted human genomic DNA. Reaction mixture was incubated at 37°C for 2min followed by 95°C for 5min, 30 cycles of 20sec at 95°C, 20sec at 55°C, 30sec at 72°C. Accupower® PCR PreMix(with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP. The amount of DNA used to test is represented below.
Lane 1: 10ng of Human genomic DNA Lane 2: 1ng of Human genomic DNA
Lane 3: 100pg of Human genomic DNA Lane 4: 10pg of Human genomic DNA
M: 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)

Figure 2. Comparison of amplification quality using PCR products(not including uracil base) between Accupower® PCR PreMix and Accupower® PCR PreMix(with UDG).
Amplification quality test was operated using serial diluted PCR products not including uracil base. Accupower® PCR PreMix was also tested for negative control. Reaction mixture was incubated at 37°C for 2min, followed by 95°C for 5min, 30 cycles of 20sec at 95°C, 20sec at 55°C, 30sec at 72°C. Accupower® PCR PreMix(with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP. The copy number of PCR products(copy) used to test is represented below.
Lane 1: 10¹¹copy    Lane 2: 10¹⁰copy    Lane 3: 10⁹copy
Lane 4: 10⁸copy      Lane 5: 10⁷copy      Lane 6: 10⁶copy
Lane 7: 10⁵copy      Lane 8: 10⁴copy      Lane 9: 10³copy
Lane 10: 10²copy    Lane N: No template control
M: 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)

 

 Prevention of carryover contamination

Figure 3. Efficiency of uracil DNA glycosylase using PCR products(including uracil base).
Efficiency test of uracil DNA glycosylase was operated using serial diluted PCR products including uracil base. Accupower® PCR PreMix was also tested for negative control.
Reaction mixture was incubated at 37°C for 2min, followed by 95°C for 5min, 30 cycles of 20sec at 95°C, 20sec at 55°C, 30sec at 72°C. Accupower® PCR PreMix(with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP.
The copy number of PCR products used to test is represented below.
Lane 1: 10¹¹copy     Lane 2: 10¹⁰copy    Lane 3: 10⁹copy
Lane 4: 10⁸copy      Lane 5: 10⁷copy      Lane 6: 10⁶copy
Lane 7: 10⁵copy      Lane 8: 10⁴copy      Lane 9: 10³copy
Lane 10: 10²copy    Lane N : No template control M: 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)