cDNA synthesis followed by PCR in one tube

 

Specifications

 

5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: No
Fragment size: 6 kb

 

Application

 

cDNA synthesis followed by PCR

 

 

 

 

Figure 1. Specific amplification of 5' - UTR region of HCV with AccuPower® RT-PCR PreMix.
Human DNA was amplified to generate 0.1-1.5 kb PCR fragments using 1 U of polymerase. The comparison was performed under the same experimental conditions.

 

 

 

Lane 1: DNA Ladder (BM corp.)
Lane 2: Negative control
Lane 3, 4: HCV positive serum
Lane 5: HCV negative serum

 

Figure 2. Sensitivity comparison between AccuPower® RT/PCR PreMix and Supplier I RT-PCR Kit
Each 10⁹ copy – 10³ copy of PSTVd used for RT/PCR and the same amount of RT/PCR products used for electrophoresis

 

 

 

Lane1: 10⁹ copy     Lane2: 10⁸ copy
Lane3: 10⁷ copy     Lane4: 10⁶ copy
Lane5: 10⁵ copy     Lane6: 10⁴ copy
Lane7: 10³ copy     Lane8: NTC

 

Figure 3. Comparison of reproducibility test for AccuPower RT/PCR PreMix batch 1,2,3 and batch 4 products using serial diluted Human Total RNA.
Each 10⁹ copy – 10³ copy of PSTVd used for RT/PCR and the same amount of RT/PCR products used for electrophoresis

 

 

 

Lane 1: 10 ng Human total RNA from HeLa cell
Lane 2: 1 ng Human total RNA from HeLa cell
Lane 3: 100 pg Human total RNA from HeLa cell
Lane 4: 10 pg Human total RNA from HeLa cell