Enzyme Properties
5' to 3' exonuclease activity: Yes
3' to 5' exonuclease activity: No
3’ – A Overhang: Yes
Fragment Size: -
Application
- Gene expression profiling
- Target DNA quantification
- Microbial detection
- Viral/bacterial pathogen load determination
- Evaluation of primer pair performance for probe-based Real-Time PCR
Storage Temperature
-20°C
Experimental Data
Figure 1. High specificity of AccuPower® Plus DualStar™ qPCR PreMix
Figure 2. Comparison of amplification quality between AccuPower® Plus DualStar™ qPCR PreMix and other supplier's Real time qPCR kit.
Figure 3. Five-target multiplexing on the Exicycler™ instrument using AccuPower® Plus DualStar™ qPCR PreMix
Figure 3 shows amplification of a 5-target multiplex assay. The dyes used were FAM, TET, CY5, Texas Red and TAMRA, respectively. The data demonstrate that over a dilution series of input template, the AccuPower® Plus DualStar™ qPCR PreMix can successfully and reliably generate up to 5-target multiplex data on the Exicycler™.
Figure 4. PCR inhibitor study using AccuPower® Plus DualStar™ qPCR PreMix