Performance of miRNA mimic Positive and Negative controls
Figure1. AccuTarget miRNA Positive & Negative controls were transfected at 20 nM using Lipofectamine RNAiMAX into HeLa cell lines and assessed for their ability to decrease target mRNA levels.
Down-regulation of GAPDH was determined using the real-time quantitative RT-PCR at 48 hours post-transfection using Bioneer’s Exicycler 96 qPCR instrument.