RNA Phosphoramidites from Bioneer
For RNA oligonucleotides synthesis and more
Overview
Ordering Information
Features of RNA Phosphoramidites
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Amine groups are protected by protecting groups {rA(Bz), rC(Ac) rG(Ib)}. |
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The recommended procedure for cleavage and deprotection for standard oligonucleotide synthesis is treatment with concentrated ammonia for 8 hours at 55°C or by the use of methylamine gas. |
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Cleavage and deprotection procedures for RNA synthesis are similar to DNA synthesis, with an additional step to remove the 2'-OH protecting group. |
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The 2'-OH group is protected by a tert-butyldimethylsilyl (TBDMS) group to prevent derivatization and degradation during the synthesis cycle. |
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Standard RNA phosphoramidites provide excellent coupling efficiency when used together with either ETT or BTT as an activator. |
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The purities of manufactured nucleoside phosphoramidites are quality controlled via RP-HPLC/31P-NMR analysis. (RP- HPLC: ≥ 99%, 31P-NMR: ≥ 99%) |
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All reagents are conveniently packaged for ABI instruments. Expedite- and Mermade-compatible vials are also available upon request. |
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Fig 1. rG(Ib)-Phosphoramidite HPLC Data
RP- HPLC : ≥ 99% |
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Fig 2. rG(Ib)-Phosphoramidite 31P-NMR Data
31P-NMR : ≥ 99% |