Specifications

Column loading volume	800 µl
Max binding capacity	~ 120 µg
Elution volume	30 µl - 100 µl
Preparation time	~ 20 min

 

Sample	Amount	Typical yield	Purity
Cultured cells	104 – 108 cells	15 – 20 μg	A260/280 > 2.0 A260/230 > 1.7
Mammalian tissue	25 – 50 mg	10 - 60 μg
Plant tissue	100 mg	30 - 60 μg

* Differences in the quantified value may occur depending on the types of samples.

Components

Components	K-3141 (50 reactions)	K-3140 (100 reactions)
RB Buffer (RNA Binding)	30 ml x 1 ea	60 ml x 1 ea
RWA1 Buffer (1st Washing)	40 ml x 1 ea	80 ml x 1 ea
RWA2 Buffer (2nd Washing)	70 ml x 1 ea	70 ml x 2 ea
ER Buffer (Elution)	10 ml x 1 ea	20 ml x 1 ea
AccuPrep® Binding Column-III	50 ea	100 ea
1.5 ml Tube (for collection)	50 ea	100 ea
User's Guide	1	1

 

Experimental Data

Figure 1. Cultured cell RNA extraction using AccuPrep^® Universal RNA Extraction Kit
RNA extraction from 1×10⁶ of HeLa cell. DNase was not treated.
1: Bioneer, 2: Company Q

Figure 2. Plant RNA extraction using AccuPrep^® Universal RNA Extraction Kit
RNA extraction from 100 mg of Brassica oleracea var. italica. DNase was not treated.
1: Bioneer, 2: Company Q

Figure 3. Animal tissue RNA extraction using AccuPrep^® Universal RNA Extraction Kit
RNA extraction from 20 mg of Mus musculus liver. DNase was not treated.
1: Bioneer, 2: Company Q