Bioneer’s AccuPrep Plasmid MiniPrep DNA Extraction Kit allows for the extraction of highly-pure plasmid
DNA from as E. coli bacterial cultures. A260/A280 ratio greater than 1.8 and Yields of up to 20 μg of
high-copy plasmid DNA can be routinely attained using the AccuPrep Plasmid MiniPrep DNA Extraction Kit.
The AccuPrep Plasmid MiniPrep DNA Extraction Kit was developed for plasmid DNA purification from cultured
bacterial cells within 20 minutes. The overall principle uses a modified alkaline lysis method. Collected cells
are resuspended in Resuspension Buffer. Following the addition of Lysis Buffer and Neutralization Buffer
to the lysate, the chromosomal DNA and cell debris will be forms an insoluble aggregate. The insoluble
protein aggregate is removed following centrifugation and transfer the clear lysate to the DNA binding
filter tube. The cleared lysate contains a chaotropic salt originating from Neutralization Buffer which
helps the binding of the plasmid DNA on the membrane surface. The DNA binding filter of the AccuPrep
Plasmid MiniPrep DNA Extraction Kit is packed with silica based membrane has enough surface area to
bind up to 20 μg of plasmid DNA. Any salts and precipitates are eliminated by addition of the Washing
Buffer. Finally, highly purified plasmid DNA is eluted with low salt Elution Buffer and is immediately
ready for downstream applications.
Features and Benefits
| High nucleic acid purity: |
Ideal for all downstream applications |
| High yield: |
More plasmid |
| Value pricing: |
Great value for your dollar |