AccuPower® RocketScript™ PreMix contains Bioneer’s exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript. Conditions are optimized for Bioneer’s Cyclic Temperature Reverse Transcription (CTRT; patent pending) in a premix form.
Native form of M-MLV RTase has maximum activity at relatively low temperatures of 42°C, causing several problems in reverse transcription of RNA molecules with complex secondary structure. Thus, AccuPower RocketScript Cycle RT PreMix is designed to provide thermostable activity across a wide temperature range, (42°C – 70°C), allowing efficient cDNA synthesis from virtually any RNA. With the premix is lyophilized, this product contains all components necessary for a successful CTRT reaction, including RTase, RNase inhibitor and buffer components. You would need to add only template RNA and water, and the RT reaction is ready to go.
Note: Schematic representation of the 5’UTR of a gene, with complex secondary structure, at three different temperatures. Note that RocketScript shows full activity at 70°C allowing it to synthesize the whole length of several gene sequences which M-MLV and other reverse transcriptases fail to synthesize.
Features and Benefits
Thermostable Activity:
RocketScript performs reverse transcription reactions throughout a wide range of temperatures from (42°C – 70°C).
Enhanced Performance:
RocketScript has enhanced performance to handle high and low input RNA concentrations as well as short and long
RT target sizes.
Easy-to-use:
The product contains the enzyme itself, plus RNase inhibitors and all other components necessary for the best reverse
transcription results in the tube.
Reproducibility:
Bioneer's strict quality controlled production system ensures that your results will be reproducible experiment after
experiment.
Flexible Reaction Conditions:
Both CTRT and first-strand cDNA synthesis within (42°C – 70°C) are possible.
Convenient:
Just add template and primers and start your reaction. dNTPs, buffer and enzyme are provided.
Stability:
Stable at room temperature for a month one year at 4°C and for 2 years in a -20°C freezer
RNase, DNase and Proteinase-free:
Ensures the integrity of your samples.
Broad range of working temperatures:
For RNAs that are G:C rich or significant secondary structure
Stable at room temperature for a month and for 2 years at -20°C:
Long shelf life.