Specifications
| Sample Type |
Mini |
Midi |
Maxi |
| Plant tissue |
~ 100 mg |
~ 500 mg |
~ 1 g |
| Seed |
~ 50 mg |
~ 250 mg |
~ 500 mg |
| Elution volume |
100 ul |
500 ul |
1 ml |
| Typical DNA yield |
5 ~ 15 ug |
15 ~ 80 ug |
30 ~ 150 ug |
| Expected purity |
A260/280> 1.7, 260/230 > 1.2 |
Application
Gene Cloning, PCR, Real-Time PCR, Southern Blotting, SNP genotyping
Experimental Data
Figure 1. Comparison of Genomic DNA from plant tissue purified with MagListo™ 5M Plant Genomic DNA Extraction Kit and competitor’s kit (Single Column Type)
 |
M: Bioneer 1 kb ladder
B: Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit
Q: Competitor Q kit |
Perilla: Genomic DNA from 100mg leaves of Perilla frutescens var. japonica Regel
B: mean yield; 7.1 μg, mean purity; 1.80 Q: mean yield; 3.5 μg, mean purity; 1.85
Chinese cabbage: Genomic DNA from 100mg leaves of Brassica rapa var. glabra Regel
B: mean yield; 5.9 μg, mean purity; 1.82 Q: mean yield; 4.7 μg, mean purity; 1.82
Zucchini: Genomic DNA from 100mg tissue of Cucurbita pepo
B: mean yield; 3.6 μg, mean purity; 1.77 Q: mean yield; 3.0 μg, mean purity; 1.79
Onion: Genomic DNA from 100mg tissue of Allium cepa
B: mean yield; 5.5 μg, mean purity; 1.80 Q: mean yield; 1.6 μg, mean purity; 1.84
Kidney bean: Genomic DNA from 50mg seeds of Phaseolus vulgrais
B: mean yield; 7.7 μg, mean purity; 1.73 Q: mean yield; 6.6 μg, mean purity; 1.80
Figure 2. Comparison of Genomic DNA from flower bud tissue of broccoli (Brassica oleracea var. italica) purified
with MagListo™ 5M Plant Genomic DNA Extraction Kit and competitor’s kit (Single Column Type)
 |
M: Bioneer 1 kb ladder
1-2: ((Mini) Genomic DNA from broccoli (100mg) purified with Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit, mean yield; 4.7 μg, mean purity; 1.85
3-4: (Mini) Genomic DNA broccoli (100mg) purified with competitor Q kit, mean yield; 3.7 μg, mean purity; 1.84
5-6: (Midi) Genomic DNA from broccoli (500mg), mean yield; 25.9 μg, mean purity; 1.86
7-8: (Maxi) Genomic DNA from broccoli (1g), mean yield; 48.2 μg, mean purity; 1.84 |
Figure 3. Comparison of Genomic DNA from Cucumis sativus (cucumber) seed purified with MagListo™ 5M Plant Genomic DNA Extraction Kit and competitor’s kit (Single Column Type)
 |
M: Bioneer 1 kb ladder
1-2: ((Mini) Genomic DNA from cucumber seed (50mg) purified with Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit, mean yield; 2.8 μg, mean purity; 1.68
3-4: (Mini) Genomic DNA from cucumber seed (50mg) purified with competitor Q kit, mean yield; 1.9 μg, mean purity; 1.83
5-6: (Midi) Genomic DNA from cucumber seed (250mg), mean yield; 12.45 μg, mean purity; 1.69
7: (Maxi) Genomic DNA from cucumber seed (500mg), yield; 20.8 μg, purity; 1.71 |
Figure 4. Electrophoresis data of conventional PCR of plant Genomic DNA purified with MagListo™ 5M Genomic DNA Extraction Kit
and competitor’s kit (Single Column Type)
 |
M: Bioneer 100bp ladder
1-2: 100bp fragment of EMBRYONIC FLOWER 2 (EMF2) gene from broccoli genomic dna purified with Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit
3-4: 100bp fragment of EMBRYONIC FLOWER 2 (EMF2) gene from broccoli genomic dna purified with competitor Q kit
5-6: 180bp fragment of glyceraldehyde-3-phosphate dehydrogenase A (GAPDH) gene from cucumber seed genomic dna purified with Bioneer
MagListo™ 5M Plant Genomic DNA Extraction Kit
7-8: 180bp fragment of glyceraldehyde-3-phosphate dehydrogenase A (GAPDH) gene from cucumber genomic dna purified with competitor Q kit |