Specifications
| Sample Vol. |
15 mg |
1 ea |
20 mg |
15 mg |
| Elution Vol. |
100 ul |
100 ul |
100 ul |
100 ul |
| Yield (ug) |
25 ug |
10 ug |
8.5 ug |
4 ug |
| Purity (A260/280) |
> 1.9 |
> 1.9 |
> 1.9 |
> 1.9 |
Applications
RT-PCR, Quantitative real time RT-PCR, cDNA synthesis, Chip-array analysis
Procedure
Experimental Data
Fig. 1 Electrophoresis result with LabChip GX
Results of capillary electrophoresis of total RNA extracted from tissue sample(rat liver, 15mg) using LabChip GX(Caliper life science, USA). Average RQS(RNA Quality Score) were at least 9.4.
Fig. 2 Real-time RT-PCR results of total RNA extracted from animal tissue
Real-time RT-PCR results using an AccuPower® RocketScrpt™ RT PreMix(K-2101, Bioneer)와 AccuPower® Dualstar™ qPCR PreMix(K-6110, Bioneer) and run on ExicyclerTM 96 Real-Time Quantitative Thermal Block (A-2060, Bioneer) from total RNA extracted from liver sample (rat, 15mg).