RNA Phosphoramidites from Bioneer

For RNA oligonucleotides synthesis and more

Ordering Information

Features of RNA Phosphoramidites

- Amine groups are protected by protecting groups {rA(Bz), rC(Ac) rG(Ib)}.
- The recommended procedure for cleavage and deprotection for standard oligonucleotide synthesis is treatment with concentrated ammonia for 8 hours at 55°C or by the use of methylamine gas.
- Cleavage and deprotection procedures for RNA synthesis are similar to DNA synthesis, with an additional step to remove the 2'-OH protecting group.
- The 2'-OH group is protected by a tert-butyldimethylsilyl (TBDMS) group to prevent derivatization and degradation during the synthesis cycle.
- Standard RNA phosphoramidites provide excellent coupling efficiency when used together with either ETT or BTT as an activator.
- The purities of manufactured nucleoside phosphoramidites are quality controlled via RP-HPLC/31P-NMR analysis. (RP- HPLC: ≥ 99%, 31P-NMR: ≥ 99%)
- All reagents are conveniently packaged for ABI instruments. Expedite- and Mermade-compatible vials are also available upon request.

fig1   fig2
Fig 1. rG(Ib)-Phosphoramidite HPLC Data
RP- HPLC : ≥ 99%
Fig 2. rG(Ib)-Phosphoramidite 31P-NMR Data
31P-NMR : ≥ 99%
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