This product contains a reverse transcriptase removed with RNase activity for an effective synthesis of long size cDNAs.
- This product is shipped in dry ice.

Features and Benefits

• Long cDNA Synthesis
  Effective synthesis of cDNA up to 12.5 kb by removing the activity of RNase H
• Thermostability
  Comprehensive compatible temperature for effective cDNA synthesis according to customer's experimental conditions from 42 °C to 70 °C, with enzymes developed based on RocketScript™ Reverse Transcriptase products
• Sensitivity
  Excellent amplification sensitivity even with a trace amount of 1 pg RNA
• Reproducibility
  Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system

Application

• Gene synthesis
• First-strand synthesis of cDNA from RNA molecules (reverse transcription)
• Random priming reactions
• Library construction
• Probe labeling
• mRNA 5'-end mapping by primer extension analysis
• Real-time PCR
• RT-PCR

Experimental data

Figure. Amplification results of RocketScript Reverse Transcriptase, RNase H Minus. (A) Sensitivity (B) Thermostability.
Lane M; 100 bp DNA Ladder(Bioneer, Cat. No. D-1030), Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell, Lane 3; 1 ng human total RNA from HeLa cell
Lane 4; 100 pg human total RNA from HeLa cell, Lane 5; 10 pg human total RNA from HeLa cell