Get more cDNA in less time with CycleScript Reverse Transcriptase. CycleScript is a versatile reverse
transcriptase - applicable to both conventional Reverse Transcription and Cyclic Reverse Transcription
(Cyclic RT, patent pending – for cDNA amplification). It features high activity across a wide range of
temperatures from 37 to 55°C therefore, reverse transcription is carried out like PCR. The Cyclic RT
reaction is composed of the following steps: 1st incubation at 15~40°C for primer annealing, heating up to
42~48°C for extension, and finally incubation at 50~55°C for denaturation of the secondary structure of
the RNA (optional). Bioneer's novel Cyclic RT system offers homogeneous cDNA synthesis, with a high yield
of cDNA up to 9 kb.
Features and Benefits
| Broad range of working temperatures: |
For G:C rich RNAs or RNAs with significant secondary structure |
| Sensitive: |
Even the rarest transcript can be reliably made into cDNA |
| High yield of cDNA: |
For genes up to 9 kb within 10 minutes |
| RNase, DNase, and Proteinase-free: |
Ensures the integrity of your samples |