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HotStart PCR from Bioneer

Unique enzyme-mediated Hot Start DNA Polymerase

Overview
Technical/Specs
Literature/Support
Ordering Information

Bioneer’s HotStart DNA polymerase uses an exclusive enzyme-mediated Hot Start PCR method that, unlike most other HotStart PCR chemistries, completely releases all polymerase activity during the first denaturation step. Top DNA polymerase is completely inhibited by pyrophosphate at temperatures below 70°C. However, at temperatures above 70°C, a thermostable pyrophosphatase initiates pyrophosphate hydrolysis and activates the DNA polymerase. This prevents the formation of non-specific products and primer-dimers during the reaction set-up process and results in improved PCR specificity.


Features and Benefits

Fast: More than three times more processive than standard Taq DNA Polymerase
High performance: Amplifies fragments up to 12 kb
Value: No license fee to pay!

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