Specifications
| |
Leaf tissue (wet) |
Seed |
| |
Chinese cabbage |
Broccoli |
Chinese cabbage |
Red bean |
| Sample Vol. |
100 mg |
100 mg |
100 mg |
100 mg |
| Elution Vol. |
50 ul |
50 ul |
50 ul |
50 ul |
| Expected yield (ug) |
Up to 15 ug |
Up to 8 ug |
Up to 5 ug |
Up to 9 ug |
| Purity (A260/280) |
> 2.0 |
> 2.0 |
> 2.0 |
> 2.0 |
| Purity (A260/280) |
> 2.0 |
> 2.1 |
> 1.8 |
> 1.5 |
Applications
RT-PCR, Quantitative real time RT-PCR, cDNA synthesis, Chip-array analysis
Procedure
Experimental Data
Fig. 1 Electrophoresis result with extracted plant total RNA
Results of gel electrophoresis of total RNA extracted from various plant samples. Expected yields were on average 5-15ug depending on sample types.
Also expected purities were at least 2.0 (A260/A280) and 1.5 (A260/A230).