An Antibody-based HotStart Taq DNA Polymerase for increased specificity and robust sensitivity.

Enzyme Properties

Concentration: 5 U/ul
5' to 3' exonuclease activity: Yes
3' to 5' exonuclease activity: No
3' – A Overhang: Yes
Nuclease contamination: Certified DNase and RNase free
Extension rate: 3–10 kb/minute depending on template complexity
Fragment Size: Up to 10 kb

Application

- Multiplex PCR - Hotstart PCR - Routine PCR - Intercalating-based qPCR - Dual-labeled probe based qPCR - Real-Time quantification of DNA and cDNA targets using intercalating dye - Primer extension - TA cloning - Gene sequencing - SNP

Kit Content

Cat. No.	HotStart Taq DNA Polymerase	10 x Rxn Buffer with MgCl2	Dilution Buffer	dNTP Mixture
E-2017	250 units	0.5 ml	0.5 ml	0.5 ml
E-2017-1	1,000 units	4 x 0.5 ml	4 x 0.5 ml	4 x 0.5 ml
E-2017-2	500 units	2 x 0.5 ml	2 x 0.5 ml	2 x 0.5 ml
E-2017-3	250 units	0.5 ml	0.5 ml	-
E-2017-4	1,000 units	4 x 0.5 ml	4 x 0.5 ml	-

- 10 x Reaction Buffer: Tris-HCl, KCl, 15 mM MgCl₂, pH 9.0
- Dilution Buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stabilizers, 50 % Glycerol, pH 8.0
- dNTPs mixture: 10 mM, each dNTP 2.5 mM

Storage Conditions

HotStart Taq DNA Polymerase, including buffers and reagents, should be stored immediately upon receipt at –20°C.
If stored in the recommended temperature, this product will be stable until the expiration date printed out on the label.

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72°C.

Experimental data

Figure 1. Singleplex and multiplex PCR were carried out to amplify fragments of different sizes from human genomic DNA p53 gene.

Lane M: 100 bp DNA Ladder of Bioneer ( Cat.No D-1030)
Lane 1: 139bp fragment      Lane 5: 1,082bp fragment
Lane 2: 211bp fragment      Lane 6: 1,296bp fragment
Lane 3: 447bp fragment      Lane 7: 1,561bp fragment
Lane 4: 618bp fragment      Lane 8: Multiplex PCR of 139, 447, and 618 bp fragments

Figure 2. Performance comparison between Bioneer HotStart Taq DNA polymerase and competitors' HotStart DNA polymerase.

Lane M: 100 bp DNA Ladder of Bioneer ( Cat.No D-1030)
Lane 1: 139bp fragment      Lane 5: 1,082bp fragment
Lane 2: 211bp fragment      Lane 6: 1,296bp fragment
Lane 3: 447bp fragment      Lane 7: 1,561bp fragment
Lane 4: 618bp fragment      Lane 8: Multiplex PCR of 139, 447, and 618 bp fragments

Bioneer: Bioneer HotStart Taq DNA polymerase
A: Competitor A's HotStart DNA polymerase
B: Competitor B's HotStart DNA polymerase