Enzyme Properties
Concentration: 5 U/ul
5' to 3' exonuclease activity: Yes
3' to 5' exonuclease activity: No
3' – A Overhang: Yes
Nuclease contamination: Certified DNase and RNase free
Extension rate: 3–10 kb/minute depending on template complexity
Fragment Size: Up to 10 kb
Application
- Multiplex PCR
- Hotstart PCR
- Routine PCR
- Intercalating-based qPCR
- Dual-labeled probe based qPCR
- Real-Time quantification of DNA and cDNA targets using intercalating dye
- Primer extension
- TA cloning
- Gene sequencing
- SNP
Kit Content
| E-2017 |
250 units |
0.5 ml |
0.5 ml |
0.5 ml |
| E-2017-1 |
1,000 units |
4 x 0.5 ml |
4 x 0.5 ml |
4 x 0.5 ml |
| E-2017-2 |
500 units |
2 x 0.5 ml |
2 x 0.5 ml |
2 x 0.5 ml |
| E-2017-3 |
250 units |
0.5 ml |
0.5 ml |
- |
| E-2017-4 |
1,000 units |
4 x 0.5 ml |
4 x 0.5 ml |
- |
- 10 x Reaction Buffer: Tris-HCl, KCl, 15 mM MgCl2, pH 9.0
- Dilution Buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stabilizers, 50 % Glycerol, pH 8.0
- dNTPs mixture: 10 mM, each dNTP 2.5 mM
Storage Conditions
HotStart Taq DNA Polymerase, including buffers and reagents, should be stored immediately upon receipt at –20°C.
If stored in the recommended temperature, this product will be stable until the expiration date printed out on the label.
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72°C.
Experimental data
Figure 1. Singleplex and multiplex PCR were carried out to amplify fragments of different sizes from human genomic DNA p53 gene.
Lane M: 100 bp DNA Ladder of Bioneer ( Cat.No D-1030)
Lane 1: 139bp fragment Lane 5: 1,082bp fragment
Lane 2: 211bp fragment Lane 6: 1,296bp fragment
Lane 3: 447bp fragment Lane 7: 1,561bp fragment
Lane 4: 618bp fragment Lane 8: Multiplex PCR of 139, 447, and 618 bp fragments
Figure 2. Performance comparison between Bioneer HotStart Taq DNA polymerase and competitors' HotStart DNA polymerase.
Lane M: 100 bp DNA Ladder of Bioneer ( Cat.No D-1030)
Lane 1: 139bp fragment Lane 5: 1,082bp fragment
Lane 2: 211bp fragment Lane 6: 1,296bp fragment
Lane 3: 447bp fragment Lane 7: 1,561bp fragment
Lane 4: 618bp fragment Lane 8: Multiplex PCR of 139, 447, and 618 bp fragments
Bioneer: Bioneer HotStart Taq DNA polymerase
A: Competitor A's HotStart DNA polymerase
B: Competitor B's HotStart DNA polymerase