For cDNA Synthesis, RT-PCR, and qRT-PCR

Enzyme Properties

5' to 3' exonuclease activity: No
3' to 5' exonuclease activity: No
3’ – A Overhang: No
Strand Displacement: Yes Fragment Size: Up to 9 kb

Application

First strand cDNA synthesis from RNA, RT-PCR, and qRT-PCR

Reagents Supplied

5 x Reaction Buffer: 50 mM Tris-HCl, 250 mM KCl, 10 mM MgCl₂, pH 8.1
100 mM DTT
dNTP Mix: 2.5 mM of each dNTP

Concentration

200 U/μl

Storage Conditions

50% glycerol containing 20 mM Tris-Cl (pH7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1 % IGEPAL CA-630

Store Temperature

-20°C

Unit Definition

One unit is defined as the amount of enzyme required to incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37°C using poly (A)oligo (dT) as template primer.