AccuPower® CycleScript RT (available with: dT20, dN12, or dN6 primers) PreMix is an easy to resuspend lyophilized
PCR Master Mix of CycleScript Reverse Transcriptase, a primer, and all of the other components for cDNA synthesis
conveniently packaged in individual tubes. Simply add template RNA, and water. Then, the reverse transcription
is performed by either a Cyclic RT reaction (patent pending) or conventional reverse transcription PCR. The use
of Cyclic RT produces cDNA amplification and better results compared to conventional reverse transcription
PCR – especially for rare transcripts. The resulting cDNA can be used in a variety of applications such as
reverse transcription PCR (RT-PCR). The AccuPower® CycleScript RT (dT20, dN12, or dN6) PreMix series is stable
for 2 years at - 20°C due to a patented stabilizer.
AccuPower® CycleScript RT was developed for both a conventional reverse transcription PCR at a fixed temperature
at 42°C as well as Cyclic Reverse Transcription that is carried out like a PCR (please see the following
comparison table). Bioneer’s Cyclic RT is an innovative technology to synthesize more homogeneous cDNA in less
time compared to the conventional reverse transcription.
Conventional Reverse Transcription
| Step 1: |
RNA denaturation at 65°C for 10 minutes |
| Step 2: |
cDNA synthesis at a temperature between 37°C ~ 55°C for 15 ~ 60 minutes |
Cyclic Reverse Transcription
| Step 1: |
Primer annealing at a temperature between 15°C and 40°C for 30 seconds |
| Step 2: |
cDNA synthesis at a temperature between 42°C and 48°C for 4 minutes |
| Step 3 (optional): |
Denaturation of the secondary structure of the RNA template and cDNA synthesis at a temperature
between 50°C and 55°C for 30 seconds |
Features and Benefits
| Convenient lyophilized RT premix: |
Simply add your purified RNA and start your reaction. Enzyme, dNTPs, reaction buffer, and oligo (dT)20 are provided. |
| Broad range of working temperatures: |
For RNAs that are G:C rich or significant secondary structure |
| Novel Cyclic Reverse Transcription: |
Ensures that you isolate even the rarest transcripts |
| High yield of cDNA: |
For genes up to 9 kb within 10 minutes |
| Stable for 2 years at -20°C: |
Long life |
| RNase, DNase and Proteinase-free: |
Ensures the integrity of your samples |