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AccuPower® RocketScript™ RT PreMix from Bioneer

RocketScript™ the ONLY M-MLV Reverse Transcriptase Thermostable up to 70°C

Overview
Technical/Specs
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Ordering Information

AccuPower RocketScript RT PreMix contains BIoneer's exclusive M-MLV (Moloney Murine Leukemia Virus) based thermostable reverse transcriptase (RTase), RocketScript RTase.

Native M-MLV RTase has maximum activity at relatively low temperatures (42°C), causing several problems in reverse transcription of RNA molecules with complex secondary structure. RocketScript has thermostable activity (42-70°C), allowing efficient cDNA synthesis from virtually any RNA.
The lyophilized PreMix contains all components necessary for a successful reverse transcription reaction, including RTase, RNase inhibitor and buffer components. Just add template RNA, primers and water, and the RT reaction is ready to go.

overivew

Schematic representation of the 5’UTR of a gene, with complex secondary structure, at three different temperatures. Note that RocketScript shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other Reverse Transcriptase's fail.




Features and Benefits

Thermostable Activity: RocketScript is able to perform reverse transcription reactions throughout a wide range
of temperatures from 42°C to 70°C.

Enhanced Performance: RocketScript has enhanced performance to handle both high and low input RNA
concentrations as well as short and long RT target sizes.

Easy-to-use: The product contains the enzyme itself, plus RNase inhibitors and all other components necessary for the best reverse transcription results in the tube.

Reproducibility: Bioneer's strict quality controlled production system ensures that your results will be reproducible experiment after experiment.

Convenient: Just add template and primers and start your reaction. dNTPs, buffer and enzyme are provided.

Stability: Stable at room temperature for a month one year at 4°C and for 2 years in a -20°C freezer

RNase, DNase and Proteinase-free: Ensures the integrity of your samples.

Broad range of working temperatures: For RNAs that are G:C rich or significant secondary structure

Stable for 2 years at -20°C: Long shelf life




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