AccuPower RocketScript RT PreMix contains BIoneer's exclusive M-MLV (Moloney Murine Leukemia Virus) based thermostable reverse transcriptase (RTase),
RocketScript RTase.
Native M-MLV RTase has maximum activity at relatively low temperatures (42°C), causing several problems in reverse transcription of RNA molecules with complex secondary structure.
RocketScript has thermostable activity (42-70°C), allowing efficient cDNA synthesis from virtually any RNA.
The lyophilized PreMix contains all components necessary for a successful reverse transcription reaction,
including RTase, RNase inhibitor and buffer components.
Just add template RNA, primers and water, and the RT reaction is ready to go.
Schematic representation of the 5’UTR of a gene, with complex secondary structure, at three different temperatures. Note that RocketScript shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other Reverse Transcriptase's fail.
Features and Benefits
Thermostable Activity:
Excellent thermal resistance of RocketScript ™ RTase active even at 70℃, making it suitable for cDNA synthesis of secondary RNA structure with comprehensive selection of compatible temperature ranging from 42 to 70 ℃ depending on the required experimental condition and effective cDNA synthesis
Reproducibility:
Mass production in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product to provide reproducible experimental results
Easy of use:
Simplified protocol without the need of template RNA incubation at 70 degrees, allowing anyone to easily synthesize RNA with a large secondary structure