AccuPower RocketScript Cycle RT PreMix is a ready-to-use lyophilized mastermix containing all components for first-strand cDNA synthesis from purified Poly(A) or total RNA template.
The premix contains Bioneer's exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript, and oligo dT20 for added convenience.
Conditions are optimized for Bioneer's patented Cyclic Temperature Reverse Transcription (CTRT) in a premix form.
Native M-MLV RTase has maximum activity at relatively low temperatures (42°C), causing several problems in reverse
transcription of RNA molecules
with complex secondary structure. AccuPower RocketScript Cycle RT PreMix has thermostable activity across a wide temperature range,
(42°C – 70°C), allowing efficient cDNA synthesis from virtually any RNA.
The lyophilized PreMix contains all components necessary
for a successful CTRT reaction, including RTase, RNase inhibitor and buffer components.
Just add template RNA, primers and water, and the RT reaction is ready to go.
Schematic representation of the 5' UTR of a gene, with complex secondary structure, at three different temperatures.
Note that RocketScript shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other Reverse Transcriptase's fail.
Features and Benefits
Thermostable Activity: RocketScript is able to perform reverse transcription reactions throughout a wide range
of temperatures from 42°C to 70°C.
Enhanced Performance: RocketScript has enhanced performance to handle high and low input RNA concentrations
as well as short and long RT target sizes.
Easy-to-use: The product contains the enzyme itself, plus RNase inhibitors and all other components necessary for the best reverse transcription results in the tube.
Reproducibility: Bioneer's strict quality controlled production system ensures that your results will be reproducible experiment after experiment.
Flexible Reaction Conditions: Both CTRT and first-strand cDNA synthesis are both possible, with reaction temperatures
ranging from 42°C to 70°C.
Convenient: Just add template and primers and start your reaction. dNTPs, buffer and enzyme are provided.
Stability: Stable at room temperature for a month one year at 4°C and for 2 years in a -20°C freezer
RNase, DNase and Proteinase-free: Ensures the integrity of your samples.
Broad range of working temperatures: For RNAs that are G:C rich or significant secondary structure
Stable for 2 years at -20°C: Long shelf life