AccuPower® Dual-HotStart™ RT-PCR PreMix is a breakthrough product to overcome frequent problems from reverse transcription.
By using this proprietary product, cDNA synthesis of your selective RNA will be performed with high sensitivity from a small amount of template RNA.
Successive PCR amplification of synthesized cDNA also occurs via a one-step reaction with cDNA synthesis.
This one-step RT-PCR product is easily generated with excellent sensitivity for a wide range of applications,
including several types of virus tests and gene expression analysis experiments. This product is ready-to-use,
simply add template RNA, primers and DEPC-DW, and obtain your results with great reproducibility.
Features and Benefits
● High Sensitivity:
Low detection limit with the smallest amount of your target template RNA, even in highly concentrated sample.
● High Specificity:
Optimized to get accurate target gene amplification by using our premier Dual-HotStart™ RT-PCR reaction which utilizes
Pyro-HotStart RT reaction and HotStart PCR.
● Use of various Template RNAs:
This product includes Dual-HotStart™ RTase which can achieve RT reaction in unusually high temperature up to 70°C.
So it is possible to adopt various template RNAs even if they have a complex secondary structure.
● Ease-of-use:
This kit contains all the reagents for RT-PCR, including thermostable DNA polymerase, RocketScript™ RTase,
dNTPs and more, in one tube as freeze-and-dried format. User only needs to add template RNA,
primers and DEPC-water into the tube.
● Reproducibility:
Containing all the components in each tube or well for reverse transcription and PCR, it maximizes reproducibility
eliminating any carry-over contamination or technician error.
● Stability:
The proprietary lyophilization format allows 30 days-stable shelf life at ambient temperature and 2 years at -20°C.