ExiPrep® 48 Viral DNA/RNA Kit is designed for accurate and rapid extraction of viral DNA or RNA from various human specimens using ExiPrep™ 48 Dx .For HBV and HCV detection, a single sample can be used to detect both targets. The entire extraction process is carried out automatically via ExiPrep™ 48 Dx instrument.
Features and Benefits
- Pre-filled buffer cartridge system
- Simultaneous extraction of nucleic acids from maximum of 48 clinical samples
- ExiPrep™48 Dx system uses silica magnetic beads developed and produced with Bioneer's technology.
Specifications
| Technology | Magnetic Silica Beads |
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| Main sample type | Body fluids, Swab, Sputum |
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| Starting volume |
Serum, Plasma, Urine : 800 ul/400 ul
Whole blood : 200 ul
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| Elution volume | 50 ul |
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| Instrumention | ExiPrep™48 Dx |
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| Test | 96 |
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| Certification | CE, MFDS |
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Workflow
Performances
1.Test results using ExiPrep™ 48 Viral DNA/RNA Kit and AccuPower® HBV Quantitative PCR Kit
| HBV　 | 1.64 Log10 IU/ml (N=9) | 3.33 Log10 IU/ml (N=9) |
| Parameter | Log10 IU/ml | Log10 IU/ml |
| Avg(range) | 1.81(1.62-1.99) | 3.32(3.18-3.42) |
| SD | 0.13 | 0.09 |
| %CV | 6.98% | 2.66% |
| Bias* | 0.17 | 0.01 |
The efficiency of ExiPrep™ 48 Viral DNA/RNA Kit was confirmed with two quantitative concentrations of HBV panel. Viral DNA was extracted from serum using ExiPrep™ Viral DNA/RNA Kit and real-time PCR was performed with AccuPower® HBV Quantitative PCR Kit.
2. Test results using ExiPrep™ 48 Viral DNA/RNA Kit and AccuPower® HCV Quantitative PCR Kit.
| HCV　 | 1.48 Log10 IU/ml (N=9) | 3 Log10 IU/ml (N=9) |
| Parameter | Log10 IU/ml | Log10 IU/ml |
| Avg(range) | 1.92(1.71-2.04) | 2.91(2.84-2.97) |
| SD | 0.09 | 0.04 |
| %CV | 4.90% | 1.48% |
| Bias* | 0.44 | 0.09 |
The efficiency of ExiPrep™ 48 Viral DNA/RNA Kit was confirmed with two quantitative concentrations of HCV panel. Viral RNA was extracted from plasma using ExiPrep™ Viral DNA/RNA Kit and real-time PCR was performed with AccuPower® HCV Quantitive PCR Kit.
3. Test results using ExiPrep™ 48 Viral DNA/RNA Kit and AccuPower® CMV Quantitative PCR Kit
| CMV | 2.38 Log10 IU/ml (N=20) | 3 Log10 IU/ml (N=20) |
| Parameter | Log10 IU/ml | Log10 IU/ml |
| Avg(range) | 2.41(2.33-2.55) | 3.00(2.94-3.11) |
| SD | 0.07 | 0.05 |
| %CV | 2.95% | 1.67% |
| Bias* | 0.03 | 0 |
The efficiency of ExiPrep™ 48 Viral DNA/RNA Kit was confirmed with two quantitative concentrations of CMV panel. Viral DNA was extracted from whole blood using ExiPrep™ Viral DNA/RNA Kit and real-time PCR was performed with AccuPower® CMV Quantitative PCR Kit.