This kit is used for generating a protein without His-tag sequence with high purity on ExiProgen™.
It first synthesizes a protein with a His-tag in ExiProgen™ in an automated manner.
Then the His-tag is cleaved off, resulting in a pure protein without a His-tag sequence.
Using protocol No. 904 the target protein can be synthesized with a high purity.
All processes, including protein synthesis and dialysis, are performed in an automated manner and the final protein is harvested in any storage buffer of choice.
Features and Benefits
Protein synthesis without His-tag
A target protein is produced in a fully automated way and the His-tag is cleaved from the final protein.
Fully automated: DNA-in-Protein (in storage buffer)-out
Purified target protein will be in any storage buffer of choice.
Parallel processing and high purity
1-8 different proteins can be synthesized in a single run with purity >95%.