The easy method for protein synthesis !!

Procedure

By simply mixing E. coli extract, master mix and template DNA (prepared by PCR or cloning), your target protein will be expressed in a water bath at 30C. And the target protein is purified using Ni-NTA magnetic beads and buffer provided in the kit.


When synthesized protein is not detectable on SDS-PAGE with coomassie blue staining, you can use western blot or fluorescence for it detection.

Experimental results

Protein Synthesis using AccuRapid™ Protein Synthesis Kit

Expression sample	Purification sample

Figure 1. SDS-PAGE data of various proteins synthesized from various templates
   M; AccuLadder™ Protein Size Marker (Broad)
   1; CalmL3 (17.5 kDa), 2; DUSP 3 (22 kDa), 3; CAT (24 kDa), 4; AcGFP (29 kDa),
   5; ERFP (31 kDa), 6; EF-Ts (34 kDa), 7; VF (45 kDa), 8; BM3 (117 kDa)