Procedure and principle
This kit is designed for automated large scale expression of a target protein using patented SECF technology using ExiProgen™.
It utilizes cell-free protein synthesis and purification using Ni-NTA magnetic beads.
As it provides energy source in a stepwise manner into reaction tubes, the yields are maximized.
As described above, template DNA is mixed in a reaction tube with E. coli extract and Master mix.
The reaction tube is installed into ExiProgen™' s reaction block along with other reagents. Finally, choose protocol and initiate run.
ExiProgen™ begins to synthesize the target protein by providing the energy source in stepwise manner. The synthesized target protein is then purified via Ni-NTA magnetic beads.
Experimental results
1. Up to eight proteins with various sizes can be synthesized in a single run.
M1; AccuLadder™ Protein Size Marker (Low)
1; CalmL3 (17.5kDa) , 2; DUSP 3 (22kDa),
3; CAT (24kDa), 4; AcGFP (29kDa),
5; RFP(30 kDa) 6; EF-Ts (34kDa),
7; VF (45kDa), 8; BM3 (117kDa)
M2; AccuLadder™ Protein Size Marker (Broad)
* Amount of loading sample is 1/80 of total sample.
Note) Bioneer highly recommends using plasmid DNA containing target gene for this kit.
Kit contents and storage conditions
This kit consists of two separate boxes, and its components are described in the following table.
| Kit 1 |
Cartridge ① |
96 well x 1ea |
at 4°C |
| Dialysis tube |
1 pack (16 ea/pack) |
| Disposable filter tip |
1 pack (8 ea/pack) |
at room temperature |
| Protection cover |
1 ea |
| Kit 2 |
Cartridge ② |
96 well x 1ea |
at -20°C
(upon receiving,
E. coli extract is
recommended to be
stored at -70°C) |
| E. coli extract |
8-tube strip (Yellow) x 1 ea |
| Master mix |
8-tube strip (Violet) x 1 ea |
| DEPC DW |
8-tube strip (White) x 1 ea |
| Storage buffer |
35 mL bottle x 2 ea |
| Positive control DNA |
1.5mL tube x 1 ea |